Coevolutionary signals across protein lineages help capture multiple protein conformations
| dc.citation.journalTitle | PNAS Early Edition | en_US |
| dc.contributor.author | Morcos, Faruck | en_US |
| dc.contributor.author | Jana, Biman | en_US |
| dc.contributor.author | Hwa, Terence | en_US |
| dc.contributor.author | Onuchic, José Nelson | en_US |
| dc.date.accessioned | 2013-12-19T22:24:09Z | en_US |
| dc.date.available | 2013-12-19T22:24:09Z | en_US |
| dc.date.issued | 2013 | en_US |
| dc.description.abstract | A long-standing problem in molecular biology is the determination of a complete functional conformational landscape of proteins. This includes not only proteins’ native structures, but also all their respective functional states, including functionally important intermediates. Here, we reveal a signature of functionally important states in several protein families, using direct coupling analysis, which detects residue pair coevolution of protein sequence composition. This signature is exploited in a protein structure-based model to uncover conformational diversity, including hidden functional configurations. We uncovered, with high resolution (mean ∼1.9 Å rmsd for nonapo structures), different functional structural states for medium to large proteins (200–450 aa) belonging to several distinct families. The combination of direct coupling analysis and the structure-based model also predicts several intermediates or hidden states that are of functional importance. This enhanced sampling is broadly applicable and has direct implications in protein structure determination and the design of ligands or drugs to trap intermediate states. | en_US |
| dc.identifier.citation | Morcos, Faruck, Jana, Biman, Hwa, Terence, et al.. "Coevolutionary signals across protein lineages help capture multiple protein conformations." <i>PNAS Early Edition,</i> (2013) National Academy of Sciences: http://dx.doi.org/10.1073/pnas.1315625110. | en_US |
| dc.identifier.doi | http://dx.doi.org/10.1073/pnas.1315625110 | en_US |
| dc.identifier.uri | https://hdl.handle.net/1911/75257 | en_US |
| dc.language.iso | eng | en_US |
| dc.publisher | National Academy of Sciences | en_US |
| dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
| dc.subject.keyword | conformational plasticity | en_US |
| dc.subject.keyword | covariation | en_US |
| dc.subject.keyword | statistical inference | en_US |
| dc.subject.keyword | molecular dynamics | en_US |
| dc.title | Coevolutionary signals across protein lineages help capture multiple protein conformations | en_US |
| dc.type | Journal article | en_US |
| dc.type.dcmi | Text | en_US |
| dc.type.publication | publisher version | en_US |