Crystal structure of the protein At3g01520, a eukaryotic universal stress protein-like protein from arabidopsis thaliana in complex with AMP

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dc.citation.firstpage1368
dc.citation.issueNumber7
dc.citation.journalTitleProteins: Structure, Function, and Bioinformatics
dc.citation.lastpage1373
dc.citation.volumeNumber83
dc.contributor.authorKim, Do Jin
dc.contributor.authorBitto, Eduard
dc.contributor.authorBingman, Craig A.
dc.contributor.authorKim, Hyun-Jung
dc.contributor.authorHan, Byung Woo
dc.contributor.authorPhillips, George N.Jr.
dc.date.accessioned2015-11-09T18:52:18Z
dc.date.available2015-11-09T18:52:18Z
dc.date.issued2015
dc.description.abstractMembers of the universal stress protein (USP) family are conserved in a phylogenetically diverse range of prokaryotes, fungi, protists, and plants and confer abilities to respond to a wide range of environmental stresses. Arabidopsis thaliana contains 44 USP domain-containing proteins, and USP domain is found either in a small protein with unknown physiological function or in an N-terminal portion of a multi-domain protein, usually a protein kinase. Here, we report the first crystal structure of a eukaryotic USP-like protein encoded from the gene At3g01520. The crystal structure of the protein At3g01520 was determined by the single-wavelength anomalous dispersion method and refined to an R factor of 21.8% (Rfree =  26.1%) at 2.5 Å resolution. The crystal structure includes three At3g01520 protein dimers with one AMP molecule bound to each protomer, comprising a Rossmann-like α/β overall fold. The bound AMP and conservation of residues in the ATP-binding loop suggest that the protein At3g01520 also belongs to the ATP-binding USP subfamily members.
dc.identifier.citationKim, Do Jin, Bitto, Eduard, Bingman, Craig A., et al.. "Crystal structure of the protein At3g01520, a eukaryotic universal stress protein-like protein from arabidopsis thaliana in complex with AMP." <i>Proteins: Structure, Function, and Bioinformatics,</i> 83, no. 7 (2015) Wiley: 1368-1373. http://dx.doi.org/10.1002/prot.24821.
dc.identifier.doihttp://dx.doi.org/10.1002/prot.24821
dc.identifier.urihttps://hdl.handle.net/1911/82040
dc.language.isoeng
dc.publisherWiley
dc.rightsThis is an open access article under the terms of the Creative Commons Attribution NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subject.keyworduniversal stress protein
dc.subject.keywordArabidopsis thaliana
dc.subject.keywordAt3g01520
dc.titleCrystal structure of the protein At3g01520, a eukaryotic universal stress protein-like protein from arabidopsis thaliana in complex with AMP
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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