XTH31, Encoding an in Vitro XEH/XET-Active Enzyme, Regulates Aluminum Sensitivity by Modulating in Vivo XET Action, Cell Wall Xyloglucan Content, and Aluminum Binding Capacity in Arabidopsis

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dc.citation.firstpage4731
dc.citation.journalTitleThe Plant Cell
dc.citation.lastpage4747
dc.citation.volumeNumber24
dc.contributor.authorZhu, Xiao Fang
dc.contributor.authorShi, Yuan Zhi
dc.contributor.authorLei, Gui Jie
dc.contributor.authorFry, Stephen C.
dc.contributor.authorZhang, Bao Cai
dc.contributor.authorZhou, Yi Hua
dc.contributor.authorBraam, Janet
dc.contributor.authorJiang, Tao
dc.contributor.authorXu, Xiao Yan
dc.contributor.authorMao, Chuan Zao
dc.contributor.authorPan, Yuan Jiang
dc.contributor.authorYang, Jian Li
dc.contributor.authorWu, Ping
dc.contributor.authorZheng, Shao Jian
dc.date.accessioned2013-01-29T20:37:07Z
dc.date.available2014-01-30T06:10:04Z
dc.date.issued2012-11
dc.description.abstractXyloglucan endohydrolase (XEH) and xyloglucan endotransglucosylase (XET) activities, encoded by xyloglucan endotransglucosylase-hydrolase (XTH) genes, are involved in cell wall extension by cutting or cutting and rejoining xyloglucan chains, respectively. However, the physiological significance of this biochemical activity remains incompletely understood. Here, we find that an XTH31 T-DNA insertion mutant, xth31, is more Al resistant than the wild type. XTH31 is bound to the plasma membrane and the encoding gene is expressed in the root elongation zone and in nascent leaves, suggesting a role in cell expansion. XTH31 transcript accumulation is strongly downregulated by Al treatment. XTH31 expression in yeast yields a protein with an in vitro XEH:XET activity ratio of >5000:1. xth31 accumulates significantly less Al in the root apex and cell wall, shows remarkably lower in vivo XET action and extractable XET activity, has a lower xyloglucan content, and exhibits slower elongation. An exogenous supply of xyloglucan significantly ameliorates Al toxicity by reducing Al accumulation in the roots, owing to the formation of an Al-xyloglucan complex in the medium, as verified by an obvious change in chemical shift of (27)Al-NMR. Taken together, the data indicate that XTH31 affects Al sensitivity by modulating cell wall xyloglucan content and Al binding capacity.
dc.embargo.terms1 year
dc.identifier.citationZhu, Xiao Fang, Shi, Yuan Zhi, Lei, Gui Jie, et al.. "XTH31, Encoding an in Vitro XEH/XET-Active Enzyme, Regulates Aluminum Sensitivity by Modulating in Vivo XET Action, Cell Wall Xyloglucan Content, and Aluminum Binding Capacity in Arabidopsis." <i>The Plant Cell,</i> 24, (2012) American Society of Plant Biologists: 4731-4747. http://dx.doi.org/10.1105/tpc.112.106039.
dc.identifier.doihttp://dx.doi.org/10.1105/tpc.112.106039
dc.identifier.urihttps://hdl.handle.net/1911/69865
dc.language.isoeng
dc.publisherAmerican Society of Plant Biologists
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
dc.titleXTH31, Encoding an in Vitro XEH/XET-Active Enzyme, Regulates Aluminum Sensitivity by Modulating in Vivo XET Action, Cell Wall Xyloglucan Content, and Aluminum Binding Capacity in Arabidopsis
dc.typeJournal article
dc.type.dcmiTexten_US
dc.type.publicationpublisher version
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