XTH31, Encoding an in Vitro XEH/XET-Active Enzyme, Regulates Aluminum Sensitivity by Modulating in Vivo XET Action, Cell Wall Xyloglucan Content, and Aluminum Binding Capacity in Arabidopsis

Simple item page
dc.citation.firstpage4731en_US
dc.citation.journalTitleThe Plant Cellen_US
dc.citation.lastpage4747en_US
dc.citation.volumeNumber24en_US
dc.contributor.authorZhu, Xiao Fangen_US
dc.contributor.authorShi, Yuan Zhien_US
dc.contributor.authorLei, Gui Jieen_US
dc.contributor.authorFry, Stephen C.en_US
dc.contributor.authorZhang, Bao Caien_US
dc.contributor.authorZhou, Yi Huaen_US
dc.contributor.authorBraam, Janeten_US
dc.contributor.authorJiang, Taoen_US
dc.contributor.authorXu, Xiao Yanen_US
dc.contributor.authorMao, Chuan Zaoen_US
dc.contributor.authorPan, Yuan Jiangen_US
dc.contributor.authorYang, Jian Lien_US
dc.contributor.authorWu, Pingen_US
dc.contributor.authorZheng, Shao Jianen_US
dc.date.accessioned2013-01-29T20:37:07Zen_US
dc.date.available2014-01-30T06:10:04Zen_US
dc.date.issued2012-11en_US
dc.description.abstractXyloglucan endohydrolase (XEH) and xyloglucan endotransglucosylase (XET) activities, encoded by xyloglucan endotransglucosylase-hydrolase (XTH) genes, are involved in cell wall extension by cutting or cutting and rejoining xyloglucan chains, respectively. However, the physiological significance of this biochemical activity remains incompletely understood. Here, we find that an XTH31 T-DNA insertion mutant, xth31, is more Al resistant than the wild type. XTH31 is bound to the plasma membrane and the encoding gene is expressed in the root elongation zone and in nascent leaves, suggesting a role in cell expansion. XTH31 transcript accumulation is strongly downregulated by Al treatment. XTH31 expression in yeast yields a protein with an in vitro XEH:XET activity ratio of >5000:1. xth31 accumulates significantly less Al in the root apex and cell wall, shows remarkably lower in vivo XET action and extractable XET activity, has a lower xyloglucan content, and exhibits slower elongation. An exogenous supply of xyloglucan significantly ameliorates Al toxicity by reducing Al accumulation in the roots, owing to the formation of an Al-xyloglucan complex in the medium, as verified by an obvious change in chemical shift of (27)Al-NMR. Taken together, the data indicate that XTH31 affects Al sensitivity by modulating cell wall xyloglucan content and Al binding capacity.en_US
dc.embargo.terms1 yearen_US
dc.identifier.citationZhu, Xiao Fang, Shi, Yuan Zhi, Lei, Gui Jie, et al.. "XTH31, Encoding an in Vitro XEH/XET-Active Enzyme, Regulates Aluminum Sensitivity by Modulating in Vivo XET Action, Cell Wall Xyloglucan Content, and Aluminum Binding Capacity in Arabidopsis." <i>The Plant Cell,</i> 24, (2012) American Society of Plant Biologists: 4731-4747. http://dx.doi.org/10.1105/tpc.112.106039.en_US
dc.identifier.doihttp://dx.doi.org/10.1105/tpc.112.106039en_US
dc.identifier.urihttps://hdl.handle.net/1911/69865en_US
dc.language.isoengen_US
dc.publisherAmerican Society of Plant Biologistsen_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.titleXTH31, Encoding an in Vitro XEH/XET-Active Enzyme, Regulates Aluminum Sensitivity by Modulating in Vivo XET Action, Cell Wall Xyloglucan Content, and Aluminum Binding Capacity in Arabidopsisen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
XTH31-Encoding.pdf
Size:
2.31 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Biochemistry and Cell Biology Publications
Faculty Publications