Improvement of NADPH bioavailability in Escherichia coli through the use of phosphofructokinase deficient strains

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dc.citation.firstpage6883
dc.citation.issueNumber15
dc.citation.journalTitleApplied Microbiology and Biotechnology
dc.citation.lastpage6893
dc.citation.volumeNumber97
dc.contributor.authorWang, Yipeng
dc.contributor.authorSan, Ka-Yiu
dc.contributor.authorBennett, George N.
dc.date.accessioned2013-04-17T15:17:30Z
dc.date.available2014-04-18T05:10:04Z
dc.date.issued2013
dc.description.abstractNADPH-dependent reactions play important roles in production of industrially valuable compounds. In this study, we used phosphofructokinase (PFK)-deficient strains to direct fructose-6-phosphate to be oxidized through the pentose phosphate pathway (PPP) to increase NADPH generation. pfkA or pfkB single deletion and double-deletion strains were tested for their ability to produce lycopene. Since lycopene biosynthesis requires many NADPH, levels of lycopene were compared in a set of isogenic strains, with the pfkA single deletion strain showing the highest lycopene yield. Using another NADPH-requiring process, a one-step reduction reaction of 2-chloroacrylate to 2-chloropropionic acid by 2- haloacrylate reductase, the pfkA pfkB double-deletion strain showed the highest yield of 2-chloropropionic acid product. The combined effect of glucose-6-phosphate dehydrogenase overexpression or lactate dehydrogenase deletion with PFK deficiency on NADPH bioavailability was also studied. The results indicated that the flux distribution of fructose-6- phosphate between glycolysis and the pentose phosphate pathway determines the amount of NAPDH available for reductive biosynthesis.
dc.embargo.terms1 year
dc.identifier.citationWang, Yipeng, San, Ka-Yiu and Bennett, George N.. "Improvement of NADPH bioavailability in Escherichia coli through the use of phosphofructokinase deficient strains." <i>Applied Microbiology and Biotechnology,</i> 97, no. 15 (2013) Springer-Verlag: 6883-6893. http://dx.doi.org/10.1007/s00253-013-4859-0.
dc.identifier.doihttp://dx.doi.org/10.1007/s00253-013-4859-0
dc.identifier.urihttps://hdl.handle.net/1911/70938
dc.language.isoeng
dc.publisherSpringer-Verlag
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
dc.subject.keywordPFK
dc.subject.keywordNADPH bioavailability
dc.subject.keywordpfkA
dc.subject.keywordpfkB
dc.subject.keywordG6PDH
dc.subject.keywordE. coli
dc.titleImprovement of NADPH bioavailability in Escherichia coli through the use of phosphofructokinase deficient strains
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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