Improvement of NADPH bioavailability in Escherichia coli through the use of phosphofructokinase deficient strains

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dc.citation.firstpage6883en_US
dc.citation.issueNumber15en_US
dc.citation.journalTitleApplied Microbiology and Biotechnologyen_US
dc.citation.lastpage6893en_US
dc.citation.volumeNumber97en_US
dc.contributor.authorWang, Yipengen_US
dc.contributor.authorSan, Ka-Yiuen_US
dc.contributor.authorBennett, George N.en_US
dc.date.accessioned2013-04-17T15:17:30Zen_US
dc.date.available2014-04-18T05:10:04Zen_US
dc.date.issued2013en_US
dc.description.abstractNADPH-dependent reactions play important roles in production of industrially valuable compounds. In this study, we used phosphofructokinase (PFK)-deficient strains to direct fructose-6-phosphate to be oxidized through the pentose phosphate pathway (PPP) to increase NADPH generation. pfkA or pfkB single deletion and double-deletion strains were tested for their ability to produce lycopene. Since lycopene biosynthesis requires many NADPH, levels of lycopene were compared in a set of isogenic strains, with the pfkA single deletion strain showing the highest lycopene yield. Using another NADPH-requiring process, a one-step reduction reaction of 2-chloroacrylate to 2-chloropropionic acid by 2- haloacrylate reductase, the pfkA pfkB double-deletion strain showed the highest yield of 2-chloropropionic acid product. The combined effect of glucose-6-phosphate dehydrogenase overexpression or lactate dehydrogenase deletion with PFK deficiency on NADPH bioavailability was also studied. The results indicated that the flux distribution of fructose-6- phosphate between glycolysis and the pentose phosphate pathway determines the amount of NAPDH available for reductive biosynthesis.en_US
dc.embargo.terms1 yearen_US
dc.identifier.citationWang, Yipeng, San, Ka-Yiu and Bennett, George N.. "Improvement of NADPH bioavailability in Escherichia coli through the use of phosphofructokinase deficient strains." <i>Applied Microbiology and Biotechnology,</i> 97, no. 15 (2013) Springer-Verlag: 6883-6893. http://dx.doi.org/10.1007/s00253-013-4859-0.en_US
dc.identifier.doihttp://dx.doi.org/10.1007/s00253-013-4859-0en_US
dc.identifier.urihttps://hdl.handle.net/1911/70938en_US
dc.language.isoengen_US
dc.publisherSpringer-Verlagen_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.subject.keywordPFKen_US
dc.subject.keywordNADPH bioavailabilityen_US
dc.subject.keywordpfkAen_US
dc.subject.keywordpfkBen_US
dc.subject.keywordG6PDHen_US
dc.subject.keywordE. colien_US
dc.titleImprovement of NADPH bioavailability in Escherichia coli through the use of phosphofructokinase deficient strainsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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