Injectable dual-gelling cell-laden composite hydrogels for bone tissue engineering

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dc.citation.firstpage1
dc.citation.journalTitleBiomaterials
dc.citation.lastpage11
dc.citation.volumeNumber83
dc.contributor.authorVo, T.N.
dc.contributor.authorShah, S.R.
dc.contributor.authorLu, S.
dc.contributor.authorTatara, A.M.
dc.contributor.authorLee, E.J.
dc.contributor.authorRoh, T.T.
dc.contributor.authorTabata, Y.
dc.contributor.authorMikos, A.G.
dc.date.accessioned2017-05-03T21:11:46Z
dc.date.available2017-05-03T21:11:46Z
dc.date.issued2016
dc.description.abstractThe present work investigated the osteogenic potential of injectable, dual thermally and chemically gelable composite hydrogels for mesenchymal stem cell (MSC) delivery in vitro and in vivo. Composite hydrogels comprising copolymer macromers of N-isopropylacrylamide were fabricated through the incorporation of gelatin microparticles (GMPs) as enzymatically digestible porogens and sites for cellular attachment. High and low polymer content hydrogels with and without GMP loading were shown to successfully encapsulate viable MSCs and maintain their survival over 28 days in vitro. GMP incorporation was also shown to modulate alkaline phosphatase production, but enhanced hydrogel mineralization along with higher polymer content even in the absence of cells. Moreover, the regenerative capacity of 2 mm thick hydrogels with GMPs only, MSCs only, or GMPs and MSCs was evaluated in vivo in an 8 mm rat critical size cranial defect for 4 and 12 weeks. GMP incorporation led to enhanced bony bridging and mineralization within the defect at each timepoint, and direct bone-implant contact as determined by microcomputed tomography and histological scoring, respectively. Encapsulation of both GMPs and MSCs enabled hydrogel degradation leading to significant tissue infiltration and osteoid formation. The results suggest that these injectable, dual-gelling cell-laden composite hydrogels can facilitate bone ingrowth and integration, warranting further investigation for bone tissue engineering.
dc.identifier.citationVo, T.N., Shah, S.R., Lu, S., et al.. "Injectable dual-gelling cell-laden composite hydrogels for bone tissue engineering." <i>Biomaterials,</i> 83, (2016) Elsevier: 1-11. https://doi.org/10.1016/j.biomaterials.2015.12.026.
dc.identifier.doihttps://doi.org/10.1016/j.biomaterials.2015.12.026
dc.identifier.urihttps://hdl.handle.net/1911/94155
dc.language.isoeng
dc.publisherElsevier
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
dc.subject.keywordGelatin microparticles
dc.subject.keywordN-isopropylacrylamide
dc.subject.keywordCritical size cranial defect
dc.subject.keywordMesenchymal stem cells
dc.subject.keywordMineralization
dc.titleInjectable dual-gelling cell-laden composite hydrogels for bone tissue engineering
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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