Injectable dual-gelling cell-laden composite hydrogels for bone tissue engineering

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dc.citation.firstpage1en_US
dc.citation.journalTitleBiomaterialsen_US
dc.citation.lastpage11en_US
dc.citation.volumeNumber83en_US
dc.contributor.authorVo, T.N.en_US
dc.contributor.authorShah, S.R.en_US
dc.contributor.authorLu, S.en_US
dc.contributor.authorTatara, A.M.en_US
dc.contributor.authorLee, E.J.en_US
dc.contributor.authorRoh, T.T.en_US
dc.contributor.authorTabata, Y.en_US
dc.contributor.authorMikos, A.G.en_US
dc.date.accessioned2017-05-03T21:11:46Zen_US
dc.date.available2017-05-03T21:11:46Zen_US
dc.date.issued2016en_US
dc.description.abstractThe present work investigated the osteogenic potential of injectable, dual thermally and chemically gelable composite hydrogels for mesenchymal stem cell (MSC) delivery in vitro and in vivo. Composite hydrogels comprising copolymer macromers of N-isopropylacrylamide were fabricated through the incorporation of gelatin microparticles (GMPs) as enzymatically digestible porogens and sites for cellular attachment. High and low polymer content hydrogels with and without GMP loading were shown to successfully encapsulate viable MSCs and maintain their survival over 28 days in vitro. GMP incorporation was also shown to modulate alkaline phosphatase production, but enhanced hydrogel mineralization along with higher polymer content even in the absence of cells. Moreover, the regenerative capacity of 2 mm thick hydrogels with GMPs only, MSCs only, or GMPs and MSCs was evaluated in vivo in an 8 mm rat critical size cranial defect for 4 and 12 weeks. GMP incorporation led to enhanced bony bridging and mineralization within the defect at each timepoint, and direct bone-implant contact as determined by microcomputed tomography and histological scoring, respectively. Encapsulation of both GMPs and MSCs enabled hydrogel degradation leading to significant tissue infiltration and osteoid formation. The results suggest that these injectable, dual-gelling cell-laden composite hydrogels can facilitate bone ingrowth and integration, warranting further investigation for bone tissue engineering.en_US
dc.identifier.citationVo, T.N., Shah, S.R., Lu, S., et al.. "Injectable dual-gelling cell-laden composite hydrogels for bone tissue engineering." <i>Biomaterials,</i> 83, (2016) Elsevier: 1-11. https://doi.org/10.1016/j.biomaterials.2015.12.026.en_US
dc.identifier.doihttps://doi.org/10.1016/j.biomaterials.2015.12.026en_US
dc.identifier.urihttps://hdl.handle.net/1911/94155en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.en_US
dc.subject.keywordGelatin microparticlesen_US
dc.subject.keywordN-isopropylacrylamideen_US
dc.subject.keywordCritical size cranial defecten_US
dc.subject.keywordMesenchymal stem cellsen_US
dc.subject.keywordMineralizationen_US
dc.titleInjectable dual-gelling cell-laden composite hydrogels for bone tissue engineeringen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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