Interactions between mitoNEET and NAF-1 in cells

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dc.citation.articleNumbere0175796
dc.citation.issueNumber4
dc.citation.journalTitlePLoS ONE
dc.citation.volumeNumber12
dc.contributor.authorKarmi, Ola
dc.contributor.authorHolt, Sarah H.
dc.contributor.authorSong, Luhua
dc.contributor.authorTamir, Sagi
dc.contributor.authorLuo, Yuting
dc.contributor.authorBai, Fang
dc.contributor.authorAdenwalla, Ammar
dc.contributor.authorDarash-Yahana, Merav
dc.contributor.authorSohn, Yang-Sung
dc.contributor.authorJennings, Patricia A.
dc.contributor.authorAzad, Rajeev K.
dc.contributor.authorOnuchic, José Nelson
dc.contributor.authorMorcos, Faruck
dc.contributor.authorNechushtai, Rachel
dc.contributor.authorMittler, Ron
dc.contributor.orgCenter for Theoretical Biological Physics
dc.date.accessioned2017-05-03T18:24:05Z
dc.date.available2017-05-03T18:24:05Z
dc.date.issued2017
dc.description.abstractThe NEET proteins mitoNEET (mNT) and nutrient-deprivation autophagy factor-1 (NAF-1) are required for cancer cell proliferation and resistance to oxidative stress. NAF-1 and mNT are also implicated in a number of other human pathologies including diabetes, neurodegeneration and cardiovascular disease, as well as in development, differentiation and aging. Previous studies suggested that mNT and NAF-1 could function in the same pathway in mammalian cells, preventing the over-accumulation of iron and reactive oxygen species (ROS) in mitochondria. Nevertheless, it is unknown whether these two proteins directly interact in cells, and how they mediate their function. Here we demonstrate, using yeast two-hybrid, in vivo bimolecular fluorescence complementation (BiFC), direct coupling analysis (DCA), RNA-sequencing, ROS and iron imaging, and single and double shRNA lines with suppressed mNT, NAF-1 and mNT/NAF-1 expression, that mNT and NAF-1 directly interact in mammalian cells and could function in the same cellular pathway. We further show using an in vitro cluster transfer assay that mNT can transfer its clusters to NAF-1. Our study highlights the possibility that mNT and NAF-1 function as part of an iron-sulfur (2Fe-2S) cluster relay to maintain the levels of iron and Fe-S clusters under control in the mitochondria of mammalian cells, thereby preventing the activation of apoptosis and/or autophagy and supporting cellular proliferation.
dc.identifier.citationKarmi, Ola, Holt, Sarah H., Song, Luhua, et al.. "Interactions between mitoNEET and NAF-1 in cells." <i>PLoS ONE,</i> 12, no. 4 (2017) Public Library of Science: https://doi.org/10.1371/journal.pone.0175796.
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0175796
dc.identifier.urihttps://hdl.handle.net/1911/94139
dc.language.isoeng
dc.publisherPublic Library of Science
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleInteractions between mitoNEET and NAF-1 in cells
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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