Differential Effect of Culture Temperature and Specific Growth Rate on CHO Cell Behavior in Chemostat Culture

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dc.citation.firstpagee93865
dc.citation.issueNumber4
dc.citation.journalTitlePLoS One
dc.citation.volumeNumber9
dc.contributor.authorVergara, Mauricio
dc.contributor.authorBecerra, Silvana
dc.contributor.authorBerrios, Julio
dc.contributor.authorOsses, Nelson
dc.contributor.authorReyes, Juan
dc.contributor.authorRodriguez-Moya, Maria
dc.contributor.authorGonzalez, Ramon
dc.contributor.authorAltamirano, Claudia
dc.date.accessioned2014-04-14T20:56:33Z
dc.date.available2014-04-14T20:56:33Z
dc.date.issued2014
dc.description.abstractMild hypothermia condition in mammalian cell culture technology has been one of the main focuses of research for the development of breeding strategies to maximize productivity of these production systems. Despite the large number of studies that show positive effects of mild hypothermia on specific productivity of r-proteins, no experimental approach has addressed the indirect effect of lower temperatures on specific cell growth rate, nor how this condition possibly affects less specific productivity of r-proteins. To separately analyze the effects of mild hypothermia and specific growth rate on CHO cell metabolism and recombinant human tissue plasminogen activator productivity as a model system, high dilution rate (0.017 h21) and low dilution rate (0.012 h21) at two cultivation temperatures (37 and 33ﾰC) were evaluated using chemostat culture. The results showed a positive effect on the specific productivity of r-protein with decreasing specific growth rate at 33ﾰC. Differential effect was achieved by mild hypothermia on the specific productivity of r-protein, contrary to the evidence reported in batch culture. Interestingly, reduction of metabolism could not be associated with a decrease in culture temperature, but rather with a decrease in specific growth rate.
dc.identifier.citationVergara, Mauricio, Becerra, Silvana, Berrios, Julio, et al.. "Differential Effect of Culture Temperature and Specific Growth Rate on CHO Cell Behavior in Chemostat Culture." <i>PLoS One,</i> 9, no. 4 (2014) Public Library of Science: e93865. http://dx.doi.org/10.1371/journal.pone.0093865.
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0093865
dc.identifier.urihttps://hdl.handle.net/1911/75883
dc.language.isoeng
dc.publisherPublic Library of Science
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleDifferential Effect of Culture Temperature and Specific Growth Rate on CHO Cell Behavior in Chemostat Culture
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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