Phylogenetic analysis of eukaryotic NEET proteins uncovers a link between a key gene duplication event and the evolution of vertebrates

dc.citation.articleNumber42571en_US
dc.citation.journalTitleScientific Reportsen_US
dc.citation.volumeNumber7en_US
dc.contributor.authorInupakutika, Madhuri A.en_US
dc.contributor.authorSengupta, Sohamen_US
dc.contributor.authorNechushtai, Rachelen_US
dc.contributor.authorJennings, Patricia A.en_US
dc.contributor.authorOnuchic, José Nelsonen_US
dc.contributor.authorAzad, Rajeev K.en_US
dc.contributor.authorPadilla, Pamela A.en_US
dc.contributor.authorMittler, Ronen_US
dc.contributor.orgCenter for Theoretical Biological Physicsen_US
dc.date.accessioned2017-03-07T18:53:39Zen_US
dc.date.available2017-03-07T18:53:39Zen_US
dc.date.issued2017en_US
dc.description.abstractNEET proteins belong to a unique family of iron-sulfur proteins in which the 2Fe-2S cluster is coordinated by a CDGSH domain that is followed by the “NEET” motif. They are involved in the regulation of iron and reactive oxygen metabolism, and have been associated with the progression of diabetes, cancer, aging and neurodegenerative diseases. Despite their important biological functions, the evolution and diversification of eukaryotic NEET proteins are largely unknown. Here we used the three members of the human NEET protein family (CISD1, mitoNEET; CISD2, NAF-1 or Miner 1; and CISD3, Miner2) as our guides to conduct a phylogenetic analysis of eukaryotic NEET proteins and their evolution. Our findings identified the slime mold Dictyostelium discoideum’s CISD proteins as the closest to the ancient archetype of eukaryotic NEET proteins. We further identified CISD3 homologs in fungi that were previously reported not to contain any NEET proteins, and revealed that plants lack homolog(s) of CISD3. Furthermore, our study suggests that the mammalian NEET proteins, mitoNEET (CISD1) and NAF-1 (CISD2), emerged via gene duplication around the origin of vertebrates. Our findings provide new insights into the classification and expansion of the NEET protein family, as well as offer clues to the diverged functions of the human mitoNEET and NAF-1 proteins.en_US
dc.identifier.citationInupakutika, Madhuri A., Sengupta, Soham, Nechushtai, Rachel, et al.. "Phylogenetic analysis of eukaryotic NEET proteins uncovers a link between a key gene duplication event and the evolution of vertebrates." <i>Scientific Reports,</i> 7, (2017) Springer Nature: http://dx.doi.org/10.1038/srep42571.en_US
dc.identifier.doihttp://dx.doi.org/10.1038/srep42571en_US
dc.identifier.urihttps://hdl.handle.net/1911/94034en_US
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titlePhylogenetic analysis of eukaryotic NEET proteins uncovers a link between a key gene duplication event and the evolution of vertebratesen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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