Presence of glucose, xylose, and glycerol fermenting bacteria in the deep biosphere of the former Homestake gold mine, South Dakota

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dc.citation.articleNumber18en_US
dc.citation.journalTitleFrontiers in Microbiologyen_US
dc.citation.volumeNumber4en_US
dc.contributor.authorRastogi, Gurdeepen_US
dc.contributor.authorGurram, Raghu N.en_US
dc.contributor.authorBhalla, Adityaen_US
dc.contributor.authorGonzalez, Ramonen_US
dc.contributor.authorBischoff, Kenneth M.en_US
dc.contributor.authorHughes, Stephen R.en_US
dc.contributor.authorKumar, Sudhiren_US
dc.contributor.authorSani, Rajesh K.en_US
dc.date.accessioned2017-07-31T18:12:32Zen_US
dc.date.available2017-07-31T18:12:32Zen_US
dc.date.issued2013en_US
dc.description.abstractEight fermentative bacterial strains were isolated from mixed enrichment cultures of a composite soil sample collected at 1.34 km depth from the former Homestake gold mine in Lead, SD, USA. Phylogenetic analysis of their 16S rRNA gene sequences revealed that these isolates were affiliated with the phylum Firmicutes belonging to genera Bacillus and Clostridium. Batch fermentation studies demonstrated that isolates had the ability to ferment glucose, xylose, or glycerol to industrially valuable products such as ethanol and 1,3-propanediol (PDO). Ethanol was detected as the major fermentation end product in glucose-fermenting cultures at pH 10 with yields of 0.205–0.304 g of ethanol/g of glucose. While a xylose-fermenting strain yielded 0.189 g of ethanol/g of xylose and 0.585 g of acetic acid/g of xylose at the end of fermentation. At pH 7, glycerol-fermenting isolates produced PDO (0.323–0.458 g of PDO/g of glycerol) and ethanol (0.284–0.350 g of ethanol/g of glycerol) as major end products while acetic acid and succinic acid were identified as minor by-products in fermentation broths. These results suggest that the deep biosphere of the former Homestake gold mine harbors bacterial strains which could be used in bio-based production of ethanol and PDO.en_US
dc.identifier.citationRastogi, Gurdeep, Gurram, Raghu N., Bhalla, Aditya, et al.. "Presence of glucose, xylose, and glycerol fermenting bacteria in the deep biosphere of the former Homestake gold mine, South Dakota." <i>Frontiers in Microbiology,</i> 4, (2013) Frontiers Media S.A.: http://dx.doi.org/10.3389/fmicb.2013.00018.en_US
dc.identifier.digitalPresence_of_glucoseen_US
dc.identifier.doihttp://dx.doi.org/10.3389/fmicb.2013.00018en_US
dc.identifier.urihttps://hdl.handle.net/1911/95630en_US
dc.language.isoengen_US
dc.publisherFrontiers Media S.A.en_US
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/en_US
dc.subject.keyword1,3-propanediolen_US
dc.subject.keywordbioenergyen_US
dc.subject.keywordbioethanolen_US
dc.subject.keywordbiofuelsen_US
dc.subject.keywordfermentationen_US
dc.subject.keywordgold mineen_US
dc.titlePresence of glucose, xylose, and glycerol fermenting bacteria in the deep biosphere of the former Homestake gold mine, South Dakotaen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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