Presence of glucose, xylose, and glycerol fermenting bacteria in the deep biosphere of the former Homestake gold mine, South Dakota

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dc.citation.articleNumber18
dc.citation.journalTitleFrontiers in Microbiology
dc.citation.volumeNumber4
dc.contributor.authorRastogi, Gurdeep
dc.contributor.authorGurram, Raghu N.
dc.contributor.authorBhalla, Aditya
dc.contributor.authorGonzalez, Ramon
dc.contributor.authorBischoff, Kenneth M.
dc.contributor.authorHughes, Stephen R.
dc.contributor.authorKumar, Sudhir
dc.contributor.authorSani, Rajesh K.
dc.date.accessioned2017-07-31T18:12:32Z
dc.date.available2017-07-31T18:12:32Z
dc.date.issued2013
dc.description.abstractEight fermentative bacterial strains were isolated from mixed enrichment cultures of a composite soil sample collected at 1.34 km depth from the former Homestake gold mine in Lead, SD, USA. Phylogenetic analysis of their 16S rRNA gene sequences revealed that these isolates were affiliated with the phylum Firmicutes belonging to genera Bacillus and Clostridium. Batch fermentation studies demonstrated that isolates had the ability to ferment glucose, xylose, or glycerol to industrially valuable products such as ethanol and 1,3-propanediol (PDO). Ethanol was detected as the major fermentation end product in glucose-fermenting cultures at pH 10 with yields of 0.205–0.304 g of ethanol/g of glucose. While a xylose-fermenting strain yielded 0.189 g of ethanol/g of xylose and 0.585 g of acetic acid/g of xylose at the end of fermentation. At pH 7, glycerol-fermenting isolates produced PDO (0.323–0.458 g of PDO/g of glycerol) and ethanol (0.284–0.350 g of ethanol/g of glycerol) as major end products while acetic acid and succinic acid were identified as minor by-products in fermentation broths. These results suggest that the deep biosphere of the former Homestake gold mine harbors bacterial strains which could be used in bio-based production of ethanol and PDO.
dc.identifier.citationRastogi, Gurdeep, Gurram, Raghu N., Bhalla, Aditya, et al.. "Presence of glucose, xylose, and glycerol fermenting bacteria in the deep biosphere of the former Homestake gold mine, South Dakota." <i>Frontiers in Microbiology,</i> 4, (2013) Frontiers Media S.A.: http://dx.doi.org/10.3389/fmicb.2013.00018.
dc.identifier.digitalPresence_of_glucose
dc.identifier.doihttp://dx.doi.org/10.3389/fmicb.2013.00018
dc.identifier.urihttps://hdl.handle.net/1911/95630
dc.language.isoeng
dc.publisherFrontiers Media S.A.
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/
dc.subject.keyword1,3-propanediol
dc.subject.keywordbioenergy
dc.subject.keywordbioethanol
dc.subject.keywordbiofuels
dc.subject.keywordfermentation
dc.subject.keywordgold mine
dc.titlePresence of glucose, xylose, and glycerol fermenting bacteria in the deep biosphere of the former Homestake gold mine, South Dakota
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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