Cell-specific transmembrane injection of molecular cargo with gold nanoparticle-generated transient plasmonic nanobubbles

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dc.citation.firstpage5441
dc.citation.issueNumber21
dc.citation.journalTitleBiomaterials
dc.citation.lastpage5450
dc.citation.volumeNumber33
dc.contributor.authorLukianova-Hleb, Ekaterina Y.
dc.contributor.authorWagner, Daniel S.
dc.contributor.authorBrenner, Malcolm K.
dc.contributor.authorLapotko, Dmitri O.
dc.date.accessioned2015-01-06T15:49:13Z
dc.date.available2015-01-06T15:49:13Z
dc.date.issued2012
dc.description.abstractOptimal cell therapies require efficient, selective and rapid delivery of molecular cargo into target cells without compromising their viability. Achieving these goals exﾠvivo in bulk heterogeneous multi-cell systems such as human grafts is impeded by low selectivity and speed of cargo delivery and by significant damage to target and non-target cells. We have developed a cell level approach for selective and guided transmembrane injection of extracellular cargo into specific target cells using transient plasmonic nanobubbles (PNB) as cell-specific nano-injectors. As a technical platform for this method we developed a laser flow cell processing system. The PNB injection method and flow system were tested in heterogeneous cell suspensions of target and non-target cells for delivery of Dextran-FITC dye into squamous cell carcinoma HN31 cells and transfection of human T-cells with a green fluorescent protein-encoding plasmid. In both models the method demonstrated single cell type selectivity, high efficacy of delivery (96% both for HN31 cells T-cells), speed of delivery (nanoseconds) and viability of treated target cells (96% for HN31 cells and 75% for T-cells). The PNB injection method may therefore be beneficial for real time processing of human grafts without removal of physiologically important cells.
dc.identifier.citationLukianova-Hleb, Ekaterina Y., Wagner, Daniel S., Brenner, Malcolm K., et al.. "Cell-specific transmembrane injection of molecular cargo with gold nanoparticle-generated transient plasmonic nanobubbles." <i>Biomaterials,</i> 33, no. 21 (2012) Elsevier: 5441-5450. http://dx.doi.org/10.1016/j.biomaterials.2012.03.077.
dc.identifier.doihttp://dx.doi.org/10.1016/j.biomaterials.2012.03.077
dc.identifier.urihttps://hdl.handle.net/1911/78889
dc.language.isoeng
dc.publisherElsevier
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
dc.subject.keywordDNA
dc.subject.keywordgene transfer
dc.subject.keywordlaser
dc.subject.keywordgold
dc.subject.keywordnanoparticle
dc.titleCell-specific transmembrane injection of molecular cargo with gold nanoparticle-generated transient plasmonic nanobubbles
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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