Human Omental-Derived Adipose Stem Cells Increase Ovarian Cancer Proliferation, Migration, and Chemoresistance

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dc.citation.firstpagee81859en_US
dc.citation.issueNumber12en_US
dc.citation.journalTitlePLoS ONEen_US
dc.citation.volumeNumber8en_US
dc.contributor.authorNowicka, Aleksandraen_US
dc.contributor.authorMarini, Frank C.en_US
dc.contributor.authorSolley, Travis N.en_US
dc.contributor.authorElizondo, Paula B.en_US
dc.contributor.authorZhang, Yanen_US
dc.contributor.authorSharp, Hadley J.en_US
dc.contributor.authorBroaddus, Russellen_US
dc.contributor.authorKolonin, Mikhailen_US
dc.contributor.authorMok, Samuel C.en_US
dc.contributor.authorThompson, Melissa S.en_US
dc.contributor.authorWoodward, Wendy A.en_US
dc.contributor.authorLu, Karenen_US
dc.contributor.authorSalimian, Baharen_US
dc.contributor.authorNagrath, Deepaken_US
dc.contributor.authorKlopp, Ann H.en_US
dc.date.accessioned2016-02-02T21:22:53Zen_US
dc.date.available2016-02-02T21:22:53Zen_US
dc.date.issued2013en_US
dc.description.abstractObjectives: Adipose tissue contains a population of multipotent adipose stem cells (ASCs) that form tumor stroma and can promote tumor progression. Given the high rate of ovarian cancer metastasis to the omental adipose, we hypothesized that omental-derived ASC may contribute to ovarian cancer growth and dissemination. Materials and Methods: We isolated ASCs from the omentum of three patients with ovarian cancer, with (O-ASC4, O-ASC5) and without (O-ASC1) omental metastasis. BM-MSCs, SQ-ASCs, O-ASCs were characterized with gene expression arrays and metabolic analysis. Stromal cells effects on ovarian cancer cells proliferation, chemoresistance and radiation resistance was evaluated using co-culture assays with luciferase-labeled human ovarian cancer cell lines. Transwell migration assays were performed with conditioned media from O-ASCs and control cell lines. SKOV3 cells were intraperitionally injected with or without O-ASC1 to track in-vivo engraftment. Results: O-ASCs significantly promoted in vitro proliferation, migration chemotherapy and radiation response of ovarian cancer cell lines. O-ASC4 had more marked effects on migration and chemotherapy response on OVCA 429 and OVCA 433 cells than O-ASC1. Analysis of microarray data revealed that O-ASC4 and O-ASC5 have similar gene expression profiles, in contrast to O-ASC1, which was more similar to BM-MSCs and subcutaneous ASCs in hierarchical clustering. Human O-ASCs were detected in the stroma of human ovarian cancer murine xenografts but not uninvolved ovaries. Conclusions: ASCs derived from the human omentum can promote ovarian cancer proliferation, migration, chemoresistance and radiation resistance in-vitro. Furthermore, clinical O-ASCs isolates demonstrate heterogenous effects on ovarian cancer in-vitro.en_US
dc.identifier.citationNowicka, Aleksandra, Marini, Frank C., Solley, Travis N., et al.. "Human Omental-Derived Adipose Stem Cells Increase Ovarian Cancer Proliferation, Migration, and Chemoresistance." <i>PLoS ONE,</i> 8, no. 12 (2013) Public Library of Science: e81859. http://dx.doi.org/10.1371/journal.pone.0081859.en_US
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0081859en_US
dc.identifier.urihttps://hdl.handle.net/1911/88315en_US
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/en_US
dc.titleHuman Omental-Derived Adipose Stem Cells Increase Ovarian Cancer Proliferation, Migration, and Chemoresistanceen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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