Pulsed-multiline excitation for color-blind fluorescence detection

Abstract

The present invention provides a technology called Pulse-Multiline Excitation or PME. This technology provides a novel approach to fluorescence detection with application for high-throughput identification of informative SNPs, which could lead to more accurate diagnosis of inherited disease, better prognosis of risk susceptibilities, or identification of sporadic mutations. The PME technology has two main advantages that significantly increase fluorescence sensitivity: (1) optimal excitation of all fluorophores in the genomic assay and (2) “color-blind” detection, which collects considerably more light than standard wavelength resolved detection. This technology differs significantly from the current state-of-the-art DNA sequencing instrumentation, which features single source excitation and color dispersion for DNA sequence identification. Successful implementation of the PME technology will have broad application for routine usage in clinical diagnostics, forensics, and general sequencing methodologies and will have the capability, flexibility, and portability of targeted sequence variation assays for a large majority of the population.

Description
Advisor
Degree
Type
Utility patent
Keywords
Citation

Scott, Graham B. I., Kittrell, Carter W., Curl, Robert F. and Metzker, Michael L., "Pulsed-multiline excitation for color-blind fluorescence detection." Patent US7511811B2. issued 2009-03-31. Retrieved from https://hdl.handle.net/1911/79988.

Has part(s)
Forms part of
Published Version
Rights
Link to license
Citable link to this page
Collections