Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy

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dc.citation.firstpage3408
dc.citation.issueNumber18
dc.citation.journalTitleCell
dc.citation.lastpage3.43E+32
dc.citation.volumeNumber185
dc.contributor.authorLiu, Zhuohe
dc.contributor.authorLu, Xiaoyu
dc.contributor.authorVillette, Vincent
dc.contributor.authorGou, Yueyang
dc.contributor.authorColbert, Kevin L.
dc.contributor.authorLai, Shujuan
dc.contributor.authorGuan, Sihui
dc.contributor.authorLand, Michelle A.
dc.contributor.authorLee, Jihwan
dc.contributor.authorAssefa, Tensae
dc.contributor.authorZollinger, Daniel R.
dc.contributor.authorKorympidou, Maria M.
dc.contributor.authorVlasits, Anna L.
dc.contributor.authorPang, Michelle M.
dc.contributor.authorSu, Sharon
dc.contributor.authorCai, Changjia
dc.contributor.authorFroudarakis, Emmanouil
dc.contributor.authorZhou, Na
dc.contributor.authorPatel, Saumil S.
dc.contributor.authorSmith, Cameron L.
dc.contributor.authorAyon, Annick
dc.contributor.authorBizouard, Pierre
dc.contributor.authorBradley, Jonathan
dc.contributor.authorFranke, Katrin
dc.contributor.authorClandinin, Thomas R.
dc.contributor.authorGiovannucci, Andrea
dc.contributor.authorTolias, Andreas S.
dc.contributor.authorReimer, Jacob
dc.contributor.authorDieudonné, Stéphane
dc.contributor.authorSt-Pierre, François
dc.date.accessioned2022-09-08T14:39:56Z
dc.date.available2022-09-08T14:39:56Z
dc.date.issued2022
dc.description.abstractGenetically encoded voltage indicators are emerging tools for monitoring voltage dynamics with cell-type specificity. However, current indicators enable a narrow range of applications due to poor performance under two-photon microscopy, a method of choice for deep-tissue recording. To improve indicators, we developed a multiparameter high-throughput platform to optimize voltage indicators for two-photon microscopy. Using this system, we identified JEDI-2P, an indicator that is faster, brighter, and more sensitive and photostable than its predecessors. We demonstrate that JEDI-2P can report light-evoked responses in axonal termini of Drosophila interneurons and the dendrites and somata of amacrine cells of isolated mouse retina. JEDI-2P can also optically record the voltage dynamics of individual cortical neurons in awake behaving mice for more than 30 min using both resonant-scanning and ULoVE random-access microscopy. Finally, ULoVE recording of JEDI-2P can robustly detect spikes at depths exceeding 400 μm and report voltage correlations in pairs of neurons.
dc.identifier.citationLiu, Zhuohe, Lu, Xiaoyu, Villette, Vincent, et al.. "Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy." <i>Cell,</i> 185, no. 18 (2022) Elsevier: 3408-3425.e29. https://doi.org/10.1016/j.cell.2022.07.013.
dc.identifier.digital1-s2-0-S0092867422009163-main
dc.identifier.doihttps://doi.org/10.1016/j.cell.2022.07.013
dc.identifier.urihttps://hdl.handle.net/1911/113185
dc.language.isoeng
dc.publisherElsevier
dc.rightsThis is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/).
dc.titleSustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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