Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy

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dc.citation.firstpage3408en_US
dc.citation.issueNumber18en_US
dc.citation.journalTitleCellen_US
dc.citation.lastpage3.43E+32en_US
dc.citation.volumeNumber185en_US
dc.contributor.authorLiu, Zhuoheen_US
dc.contributor.authorLu, Xiaoyuen_US
dc.contributor.authorVillette, Vincenten_US
dc.contributor.authorGou, Yueyangen_US
dc.contributor.authorColbert, Kevin L.en_US
dc.contributor.authorLai, Shujuanen_US
dc.contributor.authorGuan, Sihuien_US
dc.contributor.authorLand, Michelle A.en_US
dc.contributor.authorLee, Jihwanen_US
dc.contributor.authorAssefa, Tensaeen_US
dc.contributor.authorZollinger, Daniel R.en_US
dc.contributor.authorKorympidou, Maria M.en_US
dc.contributor.authorVlasits, Anna L.en_US
dc.contributor.authorPang, Michelle M.en_US
dc.contributor.authorSu, Sharonen_US
dc.contributor.authorCai, Changjiaen_US
dc.contributor.authorFroudarakis, Emmanouilen_US
dc.contributor.authorZhou, Naen_US
dc.contributor.authorPatel, Saumil S.en_US
dc.contributor.authorSmith, Cameron L.en_US
dc.contributor.authorAyon, Annicken_US
dc.contributor.authorBizouard, Pierreen_US
dc.contributor.authorBradley, Jonathanen_US
dc.contributor.authorFranke, Katrinen_US
dc.contributor.authorClandinin, Thomas R.en_US
dc.contributor.authorGiovannucci, Andreaen_US
dc.contributor.authorTolias, Andreas S.en_US
dc.contributor.authorReimer, Jacoben_US
dc.contributor.authorDieudonné, Stéphaneen_US
dc.contributor.authorSt-Pierre, Françoisen_US
dc.date.accessioned2022-09-08T14:39:56Zen_US
dc.date.available2022-09-08T14:39:56Zen_US
dc.date.issued2022en_US
dc.description.abstractGenetically encoded voltage indicators are emerging tools for monitoring voltage dynamics with cell-type specificity. However, current indicators enable a narrow range of applications due to poor performance under two-photon microscopy, a method of choice for deep-tissue recording. To improve indicators, we developed a multiparameter high-throughput platform to optimize voltage indicators for two-photon microscopy. Using this system, we identified JEDI-2P, an indicator that is faster, brighter, and more sensitive and photostable than its predecessors. We demonstrate that JEDI-2P can report light-evoked responses in axonal termini of Drosophila interneurons and the dendrites and somata of amacrine cells of isolated mouse retina. JEDI-2P can also optically record the voltage dynamics of individual cortical neurons in awake behaving mice for more than 30 min using both resonant-scanning and ULoVE random-access microscopy. Finally, ULoVE recording of JEDI-2P can robustly detect spikes at depths exceeding 400 μm and report voltage correlations in pairs of neurons.en_US
dc.identifier.citationLiu, Zhuohe, Lu, Xiaoyu, Villette, Vincent, et al.. "Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy." <i>Cell,</i> 185, no. 18 (2022) Elsevier: 3408-3425.e29. https://doi.org/10.1016/j.cell.2022.07.013.en_US
dc.identifier.digital1-s2-0-S0092867422009163-mainen_US
dc.identifier.doihttps://doi.org/10.1016/j.cell.2022.07.013en_US
dc.identifier.urihttps://hdl.handle.net/1911/113185en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsThis is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/).en_US
dc.titleSustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopyen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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