Detection and characterization of constitutive replication origins defined by DNA polymerase epsilon

Jaksik, Roman; Wheeler, David A.; Kimmel, Marek

Detection and characterization of constitutive replication origins defined by DNA polymerase epsilon

Files

s12915-023-01527-z.pdf (4.71 MB)

Date

2023

Authors

Jaksik, Roman

Wheeler, David A.

Kimmel, Marek

Publisher

Springer Nature

Abstract

Despite the process of DNA replication being mechanistically highly conserved, the location of origins of replication (ORI) may vary from one tissue to the next, or between rounds of replication in eukaryotes, suggesting flexibility in the choice of locations to initiate replication. Lists of human ORI therefore vary widely in number and location, and there are currently no methods available to compare them. Here, we propose a method of detection of ORI based on somatic mutation patterns generated by the mutator phenotype of damaged DNA polymerase epsilon (POLE).

Type

Journal article

Citation

Jaksik, Roman, Wheeler, David A. and Kimmel, Marek. "Detection and characterization of constitutive replication origins defined by DNA polymerase epsilon." BMC Biology, 21, (2023) Springer Nature: https://doi.org/10.1186/s12915-023-01527-z.

Published Version

https://doi.org/10.1186/s12915-023-01527-z

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