Interaction of Daptomycin with Lipid Bilayers: A Lipid Extracting Effect

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dc.citation.firstpage5384en_US
dc.citation.issueNumber33en_US
dc.citation.journalTitleBiochemistryen_US
dc.citation.lastpage5392en_US
dc.citation.volumeNumber53en_US
dc.contributor.authorChen, Yen-Feien_US
dc.contributor.authorSun, Tzu-Linen_US
dc.contributor.authorSun, Yenen_US
dc.contributor.authorHuang, Huey W.en_US
dc.date.accessioned2014-09-05T20:26:38Zen_US
dc.date.available2014-09-05T20:26:38Zen_US
dc.date.issued2014en_US
dc.description.abstractDaptomycin is the first approved member of a new structural class of antibiotics, the cyclic lipopeptides. The peptide interacts with the lipid matrix of cell membranes, inducing permeability of the membrane to ions, but its molecular mechanism has been a puzzle. Unlike the ubiquitous membrane-acting host-defense antimicrobial peptides, daptomycin does not induce pores in the cell membranes. Thus, how it affects the permeability of a membrane to ions is not clear. We studied its interaction with giant unilamellar vesicles (GUVs) and discovered a lipid-extracting phenomenon that correlates with the direct action of daptomycin on bacterial membranes observed in a recent fluorescence microscopy study. Lipid extraction occurred only when the GUV lipid composition included phosphatidylglycerol and in the presence of Ca2+ ions, the same condition found to be necessary for daptomycin to be effective against bacteria. Furthermore, it occurred only when the peptide/lipid ratio exceeded a threshold value, which could be the basis of the minimal inhibitory concentration of daptomycin. In this first publication on the lipid extracting effect, we characterize its dependence on ions and lipid compositions. We also discuss possibilities for connecting the lipid extracting effect to the antibacterial activity of daptomycin.en_US
dc.identifier.citationChen, Yen-Fei, Sun, Tzu-Lin, Sun, Yen, et al.. "Interaction of Daptomycin with Lipid Bilayers: A Lipid Extracting Effect." <i>Biochemistry,</i> 53, no. 33 (2014) American Chemical Society: 5384-5392. http://dx.doi.org/10.1021/bi500779g.en_US
dc.identifier.doihttp://dx.doi.org/10.1021/bi500779gen_US
dc.identifier.urihttps://hdl.handle.net/1911/77148en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.titleInteraction of Daptomycin with Lipid Bilayers: A Lipid Extracting Effecten_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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