3-Dimensional spatially organized PEG-based hydrogels for an aortic valve co-culture model

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dc.citation.firstpage354en_US
dc.citation.journalTitleBiomaterialsen_US
dc.citation.lastpage364en_US
dc.citation.volumeNumber67en_US
dc.contributor.authorPuperi, Daniel S.en_US
dc.contributor.authorBalaoing, Liezl R.en_US
dc.contributor.authorO'Connell, Ronan W.en_US
dc.contributor.authorWest, Jennifer L.en_US
dc.contributor.authorGrande-Allen, K. Janeen_US
dc.date.accessioned2016-10-21T17:01:00Zen_US
dc.date.available2016-10-21T17:01:00Zen_US
dc.date.issued2015en_US
dc.description.abstractPhysiologically relevant inﾠvitro models are needed to study disease progression and to develop and screen potential therapeutic interventions for disease. Heart valve disease, in particular, has no early intervention or non-invasive treatment because there is a lack of understanding the cellular mechanisms which lead to disease. Here, we establish a novel, customizable synthetic hydrogel platform that can be used to study cell-cell interactions and the factors which contribute to valve disease. Spatially localized cell adhesive ligands bound in the scaffold promote cell growth and organization of valve interstitial cells and valve endothelial cells in 3D co-culture. Both cell types maintained phenotypes, homeostatic functions, and produced zonally localized extracellular matrix. This model extends the capabilities of inﾠvitro research by providing a platform to perform direct contact co-culture with cells in their physiologically relevant spatial arrangement.en_US
dc.identifier.citationPuperi, Daniel S., Balaoing, Liezl R., O'Connell, Ronan W., et al.. "3-Dimensional spatially organized PEG-based hydrogels for an aortic valve co-culture model." <i>Biomaterials,</i> 67, (2015) Elsevier: 354-364. http://dx.doi.org/10.1016/j.biomaterials.2015.07.039.en_US
dc.identifier.doihttp://dx.doi.org/10.1016/j.biomaterials.2015.07.039en_US
dc.identifier.urihttps://hdl.handle.net/1911/91982en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.en_US
dc.subject.keywordbiomimetic materialen_US
dc.subject.keywordco-cultureen_US
dc.subject.keywordendothelialisationen_US
dc.subject.keywordheart valveen_US
dc.subject.keywordhydrogelen_US
dc.subject.keywordscaffolden_US
dc.title3-Dimensional spatially organized PEG-based hydrogels for an aortic valve co-culture modelen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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