Biodegradable, phosphate-containing, dual-gelling macromers for cellular delivery in bone tissue engineering

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dc.citation.firstpage286en_US
dc.citation.journalTitleBiomaterialsen_US
dc.citation.lastpage296en_US
dc.citation.volumeNumber67en_US
dc.contributor.authorWatson, Brendan M.en_US
dc.contributor.authorVo, Tiffany N.en_US
dc.contributor.authorTatara, Alexander M.en_US
dc.contributor.authorShah, Sarita R.en_US
dc.contributor.authorScott, David W.en_US
dc.contributor.authorEngel, Paul S.en_US
dc.contributor.authorMikos, Antonios G.en_US
dc.date.accessioned2016-10-21T17:01:00Zen_US
dc.date.available2016-10-21T17:01:00Zen_US
dc.date.issued2015en_US
dc.description.abstractInjectable, biodegradable, dual-gelling macromer solutions were used to encapsulate mesenchymal stem cells (MSCs) within stable hydrogels when elevated to physiologic temperature. Pendant phosphate groups were incorporated in the N-isopropyl acrylamide-based macromers to improve biointegration and facilitate hydrogel degradation. The MSCs were shown to survive the encapsulation process, and live cells were detected within the hydrogels for up to 28 days inﾠvitro. Cell-laden hydrogels were shown to undergo significant mineralization in osteogenic medium. Cell-laden and acellular hydrogels were implanted into a critical-size rat cranial defect for 4 and 12 weeks. Both cell-laden and acellular hydrogels were shown to degrade inﾠvivo and help to facilitate bone growth into the defect. Improved bone bridging of the defect was seen with the incorporation of cells, as well as with higher phosphate content of the macromer. Furthermore, direct bone-to-hydrogel contact was observed in the majority of implants, which is not commonly seen in this model. The ability of these macromers to deliver stem cells while forming in situ and subsequently degrade while facilitating bone ingrowth into the defect makes this class of macromers a promising material for craniofacial bone tissue engineering.en_US
dc.identifier.citationWatson, Brendan M., Vo, Tiffany N., Tatara, Alexander M., et al.. "Biodegradable, phosphate-containing, dual-gelling macromers for cellular delivery in bone tissue engineering." <i>Biomaterials,</i> 67, (2015) Elsevier: 286-296. http://dx.doi.org/10.1016/j.biomaterials.2015.07.016.en_US
dc.identifier.doihttp://dx.doi.org/10.1016/j.biomaterials.2015.07.016en_US
dc.identifier.urihttps://hdl.handle.net/1911/91981en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.en_US
dc.subject.keywordhydrogelen_US
dc.subject.keywordmesenchymal stem cell encapsulationen_US
dc.subject.keywordmonoacryloxyethyl phosphateen_US
dc.subject.keywordpoly(N-isopropyl acrylamide)en_US
dc.subject.keywordrat cranial defecten_US
dc.titleBiodegradable, phosphate-containing, dual-gelling macromers for cellular delivery in bone tissue engineeringen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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