High-density sub-100-nm peptide-gold nanoparticle complexes improve vaccine presentation by dendritic cells in vitro
| dc.citation.issueNumber | 72 | en_US |
| dc.citation.journalTitle | Nanoscale Research Letters | en_US |
| dc.citation.volumeNumber | 8 | en_US |
| dc.contributor.author | Lin, Adam Yuh | en_US |
| dc.contributor.author | Lunsford, Jessica | en_US |
| dc.contributor.author | Bear, Adham Sean | en_US |
| dc.contributor.author | Young, Joseph Keith | en_US |
| dc.contributor.author | Eckels, Phillip | en_US |
| dc.contributor.author | Luo, Laureen | en_US |
| dc.contributor.author | Foster, Aaron Edward | en_US |
| dc.contributor.author | Drezek, Rebekah Anna | en_US |
| dc.date.accessioned | 2013-04-29T19:21:52Z | en_US |
| dc.date.available | 2013-04-29T19:21:52Z | en_US |
| dc.date.issued | 2013 | en_US |
| dc.description.abstract | Nanocarriers have been explored to improve the delivery of tumor antigens to dendritic cells (DCs). Gold nanoparticles are attractive nanocarriers because they are inert, non-toxic, and can be readily endocytosed by DCs. Here, we designed novel gold-based nanovaccines (AuNVs) using a simple self-assembling bottom-up conjugation method to generate high-peptide density delivery and effective immune responses with limited toxicity. AuNVs were synthesized using a self-assembling conjugation method and optimized using DC-to-splenocyte interferon-γ enzyme-linked immunosorbent spot assays. The AuNV design has shown successful peptide conjugation with approximately 90% yield while remaining smaller than 80 nm in diameter. DCs uptake AuNVs with minimal toxicity and are able to process the vaccine peptides on the particles to stimulate cytotoxic T lymphocytes (CTLs). These high-peptide density AuNVs can stimulate CTLs better than free peptides and have great potential as carriers for various vaccine types. | en_US |
| dc.embargo.terms | none | en_US |
| dc.identifier.citation | Lin, Adam Yuh, Lunsford, Jessica, Bear, Adham Sean, et al.. "High-density sub-100-nm peptide-gold nanoparticle complexes improve vaccine presentation by dendritic cells in vitro." <i>Nanoscale Research Letters,</i> 8, no. 72 (2013) Springer: http://dx.doi.org/10.1186/1556-276X-8-72. | en_US |
| dc.identifier.doi | http://dx.doi.org/10.1186/1556-276X-8-72 | en_US |
| dc.identifier.uri | https://hdl.handle.net/1911/71014 | en_US |
| dc.language.iso | eng | en_US |
| dc.publisher | Springer | en_US |
| dc.rights | This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | en_US |
| dc.rights.uri | https://creativecommons.org/licenses/by/2.0/ | en_US |
| dc.subject.keyword | vaccines | en_US |
| dc.subject.keyword | gold nanoparticles | en_US |
| dc.subject.keyword | ELISPOTs | en_US |
| dc.subject.keyword | immunotherapy | en_US |
| dc.subject.keyword | dendritic cells | en_US |
| dc.subject.keyword | self-assembled monolayer | en_US |
| dc.title | High-density sub-100-nm peptide-gold nanoparticle complexes improve vaccine presentation by dendritic cells in vitro | en_US |
| dc.type | Journal article | en_US |
| dc.type.dcmi | Text | en_US |
| dc.type.publication | publisher version | en_US |