WormGender- Open-Source Software for AutomaticCaenorhabditis elegansᅠSex Ratio Measurement

dc.citation.firstpagee0139724
dc.citation.issueNumber9
dc.citation.journalTitlePLoS ONE
dc.citation.volumeNumber10
dc.contributor.authorLabocha, Marta K.
dc.contributor.authorJung, Sang-Kyu
dc.contributor.authorAleman-Meza, Boanerges
dc.contributor.authorLiu, Zheng
dc.contributor.authorZhong, Weiwei
dc.date.accessioned2016-02-01T17:23:58Z
dc.date.available2016-02-01T17:23:58Z
dc.date.issued2015
dc.description.abstractFast and quantitative analysis of animal phenotypes is one of the major challenges of current biology. Here we report the WormGender open-source software, which is designed for accurate quantification of sex ratio in Caenorhabditis elegans. The software functions include, i) automatic recognition and counting of adult hermaphrodites and males, ii) a manual inspection feature that enables manual correction of errors, and iii) flexibility to use new training images to optimize the software for different imaging conditions. We evaluated the performance of our software by comparing manual and automated assessment of sex ratio. Our data showed that the WormGender software provided overall accurate sex ratio measurements. We further demonstrated the usage of WormGender by quantifying the high incidence of male (him) phenotype in 27 mutant strains. Mutants of nine genes (brc-1, C30G12.6, cep-1, coh-3, him-3, him-5, him-8, skr-1, unc-86) showed significant him phenotype. The WormGender is written in Java and can be installed and run on both Windows and Mac platforms. The source code is freely available together with a user manual and sample data at http://www.QuantWorm.org/. The source code and sample data are also available at http://dx.doi.org/10.6084/m9.figshare.1541248.
dc.identifier.citationLabocha, Marta K., Jung, Sang-Kyu, Aleman-Meza, Boanerges, et al.. "WormGender- Open-Source Software for AutomaticCaenorhabditis elegansᅠSex Ratio Measurement." <i>PLoS ONE,</i> 10, no. 9 (2015) Public Library of Science: e0139724. http://dx.doi.org/10.1371/journal.pone.0139724.
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0139724
dc.identifier.urihttps://hdl.handle.net/1911/88294
dc.language.isoeng
dc.publisherPublic Library of Science
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleWormGender- Open-Source Software for AutomaticCaenorhabditis elegansᅠSex Ratio Measurement
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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