WormGender- Open-Source Software for AutomaticCaenorhabditis elegansﾠSex Ratio Measurement

Simple item page
dc.citation.firstpagee0139724en_US
dc.citation.issueNumber9en_US
dc.citation.journalTitlePLoS ONEen_US
dc.citation.volumeNumber10en_US
dc.contributor.authorLabocha, Marta K.en_US
dc.contributor.authorJung, Sang-Kyuen_US
dc.contributor.authorAleman-Meza, Boanergesen_US
dc.contributor.authorLiu, Zhengen_US
dc.contributor.authorZhong, Weiweien_US
dc.date.accessioned2016-02-01T17:23:58Zen_US
dc.date.available2016-02-01T17:23:58Zen_US
dc.date.issued2015en_US
dc.description.abstractFast and quantitative analysis of animal phenotypes is one of the major challenges of current biology. Here we report the WormGender open-source software, which is designed for accurate quantification of sex ratio in Caenorhabditis elegans. The software functions include, i) automatic recognition and counting of adult hermaphrodites and males, ii) a manual inspection feature that enables manual correction of errors, and iii) flexibility to use new training images to optimize the software for different imaging conditions. We evaluated the performance of our software by comparing manual and automated assessment of sex ratio. Our data showed that the WormGender software provided overall accurate sex ratio measurements. We further demonstrated the usage of WormGender by quantifying the high incidence of male (him) phenotype in 27 mutant strains. Mutants of nine genes (brc-1, C30G12.6, cep-1, coh-3, him-3, him-5, him-8, skr-1, unc-86) showed significant him phenotype. The WormGender is written in Java and can be installed and run on both Windows and Mac platforms. The source code is freely available together with a user manual and sample data at http://www.QuantWorm.org/. The source code and sample data are also available at http://dx.doi.org/10.6084/m9.figshare.1541248.en_US
dc.identifier.citationLabocha, Marta K., Jung, Sang-Kyu, Aleman-Meza, Boanerges, et al.. "WormGender- Open-Source Software for AutomaticCaenorhabditis elegansﾠSex Ratio Measurement." <i>PLoS ONE,</i> 10, no. 9 (2015) Public Library of Science: e0139724. http://dx.doi.org/10.1371/journal.pone.0139724.en_US
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0139724en_US
dc.identifier.urihttps://hdl.handle.net/1911/88294en_US
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.titleWormGender- Open-Source Software for AutomaticCaenorhabditis elegansﾠSex Ratio Measurementen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
journal.pone.0139724.pdf
Size:
923.08 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Faculty Publications
BioSciences Publications