A Simple Nematode Infection Model for Studying Candida albicans Pathogenesis

Simple item page
dc.citation.articleNumbere114
dc.citation.issueNumber1
dc.citation.journalTitleCurrent Protocols in Microbiology
dc.citation.volumeNumber59
dc.contributor.authorKim, Grace H.
dc.contributor.authorRosiana, Sierra
dc.contributor.authorKirienko, Natalia V.
dc.contributor.authorShapiro, Rebecca S.
dc.date.accessioned2020-12-07T18:56:25Z
dc.date.available2020-12-07T18:56:25Z
dc.date.issued2020
dc.description.abstractCandida albicans is an opportunistic fungal pathogen and a model organism to study fungal pathogenesis. It exists as a harmless commensal organism and member of the healthy human microbiome, but can cause life‐threatening mucosal and systemic infections. A model host to study C. albicans infection and pathogenesis is the nematode Caenorhabditis elegans. C. elegans is frequently used as a model host to study microbial‐host interactions because it can be infected by many human pathogens and there are also close morphological resemblances between the intestinal cells of C. elegans and mammals, where C. albicans infections can occur. This article outlines a detailed methodology for exploiting C. elegans as a host to study C. albicans infection, including a C. elegans egg preparation protocol and an agar‐based C. elegans killing protocol to monitor fungal virulence. These protocols can additionally be used to study C. albicans genetic mutants in order to further our understanding of the genes involved in pathogenesis and virulence in C. albicans and the mechanisms of host‐microbe interactions.
dc.identifier.citationKim, Grace H., Rosiana, Sierra, Kirienko, Natalia V., et al.. "A Simple Nematode Infection Model for Studying Candida albicans Pathogenesis." <i>Current Protocols in Microbiology,</i> 59, no. 1 (2020) Wiley: https://doi.org/10.1002/cpmc.114.
dc.identifier.doihttps://doi.org/10.1002/cpmc.114
dc.identifier.urihttps://hdl.handle.net/1911/109630
dc.language.isoeng
dc.publisherWiley
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley.
dc.titleA Simple Nematode Infection Model for Studying Candida albicans Pathogenesis
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
CP-20-0172R1_Proof_hi.pdf
Size:
842.01 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Faculty Publications
BioSciences Publications