A Simple Nematode Infection Model for Studying Candida albicans Pathogenesis

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dc.citation.articleNumbere114en_US
dc.citation.issueNumber1en_US
dc.citation.journalTitleCurrent Protocols in Microbiologyen_US
dc.citation.volumeNumber59en_US
dc.contributor.authorKim, Grace H.en_US
dc.contributor.authorRosiana, Sierraen_US
dc.contributor.authorKirienko, Natalia V.en_US
dc.contributor.authorShapiro, Rebecca S.en_US
dc.date.accessioned2020-12-07T18:56:25Zen_US
dc.date.available2020-12-07T18:56:25Zen_US
dc.date.issued2020en_US
dc.description.abstractCandida albicans is an opportunistic fungal pathogen and a model organism to study fungal pathogenesis. It exists as a harmless commensal organism and member of the healthy human microbiome, but can cause life‐threatening mucosal and systemic infections. A model host to study C. albicans infection and pathogenesis is the nematode Caenorhabditis elegans. C. elegans is frequently used as a model host to study microbial‐host interactions because it can be infected by many human pathogens and there are also close morphological resemblances between the intestinal cells of C. elegans and mammals, where C. albicans infections can occur. This article outlines a detailed methodology for exploiting C. elegans as a host to study C. albicans infection, including a C. elegans egg preparation protocol and an agar‐based C. elegans killing protocol to monitor fungal virulence. These protocols can additionally be used to study C. albicans genetic mutants in order to further our understanding of the genes involved in pathogenesis and virulence in C. albicans and the mechanisms of host‐microbe interactions.en_US
dc.identifier.citationKim, Grace H., Rosiana, Sierra, Kirienko, Natalia V., et al.. "A Simple Nematode Infection Model for Studying Candida albicans Pathogenesis." <i>Current Protocols in Microbiology,</i> 59, no. 1 (2020) Wiley: https://doi.org/10.1002/cpmc.114.en_US
dc.identifier.doihttps://doi.org/10.1002/cpmc.114en_US
dc.identifier.urihttps://hdl.handle.net/1911/109630en_US
dc.language.isoengen_US
dc.publisherWileyen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley.en_US
dc.titleA Simple Nematode Infection Model for Studying Candida albicans Pathogenesisen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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