Application of programmable bio-nano-chip system for the quantitative detection of drugs of abuse in oral fluids

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dc.citation.firstpage306en_US
dc.citation.journalTitleDrug and Alcohol Dependenceen_US
dc.citation.lastpage313en_US
dc.citation.volumeNumber153en_US
dc.contributor.authorChristodoulides, Nicolaosen_US
dc.contributor.authorDe La Garza, Richard IIen_US
dc.contributor.authorSimmons, Glennon W.en_US
dc.contributor.authorMcRae, Michael P.en_US
dc.contributor.authorWong, Jorgeen_US
dc.contributor.authorNewton, Thomas F.en_US
dc.contributor.authorSmith, Reginaen_US
dc.contributor.authorMahoney, James J. IIIen_US
dc.contributor.authorHohenstein, Justinen_US
dc.contributor.authorGomez, Sobeydaen_US
dc.contributor.authorFloriano, Pierre N.en_US
dc.contributor.authorTalavera, Humbertoen_US
dc.contributor.authorSloan, Daniel J.en_US
dc.contributor.authorMoody, David E.en_US
dc.contributor.authorAndrenyak, David M.en_US
dc.contributor.authorKosten, Thomas R.en_US
dc.contributor.authorHaque, Ahmeden_US
dc.contributor.authorMcDevitt, John T.en_US
dc.date.accessioned2017-05-30T17:03:41Zen_US
dc.date.available2017-05-30T17:03:41Zen_US
dc.date.issued2015en_US
dc.description.abstractObjective: There is currently a gap in on-site drug of abuse monitoring. Current detection methods involve invasive sampling of blood and urine specimens, or collection of oral fluid, followed by qualitative screening tests using immunochromatographic cartridges. While remote laboratories then may provide confirmation and quantitative assessment of a presumptive positive, this instrumentation is expensive and decoupled from the initial sampling making the current drug-screening program inefficient and costly. The authors applied a noninvasive oral fluid sampling approach integrated with the in-development chip-based Programmable bio-nano-chip (p-BNC) platform for the detection of drugs of abuse. Method: The p-BNC assay methodology was applied for the detection of tetrahydrocannabinol, morphine, amphetamine, methamphetamine, cocaine, methadone and benzodiazepines, initially using spiked buffered samples and, ultimately, using oral fluid specimen collected from consented volunteers. Results: Rapid (∼10 min), sensitive detection (∼ng/mL) and quantitation of 12 drugs of abuse was demonstrated on the p-BNC platform. Furthermore, the system provided visibility to time-course of select drug and metabolite profiles in oral fluids; for the drug cocaine, three regions of slope were observed that, when combined with concentration measurements from this and prior impairment studies, information about cocaine-induced impairment may be revealed. Conclusions: This chip-based p-BNC detection modality has significant potential to be used in the future by law enforcement officers for roadside drug testing and to serve a variety of other settings, including outpatient and inpatient drug rehabilitation centers, emergency rooms, prisons, schools, and in the workplace.en_US
dc.identifier.citationChristodoulides, Nicolaos, De La Garza, Richard II, Simmons, Glennon W., et al.. "Application of programmable bio-nano-chip system for the quantitative detection of drugs of abuse in oral fluids." <i>Drug and Alcohol Dependence,</i> 153, (2015) Elsevier: 306-313. http://dx.doi.org/10.1016/j.drugalcdep.2015.04.026.en_US
dc.identifier.doihttp://dx.doi.org/10.1016/j.drugalcdep.2015.04.026en_US
dc.identifier.urihttps://hdl.handle.net/1911/94741en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.en_US
dc.subject.keyworddrugs of abuseen_US
dc.subject.keywordprogrammable bio-nano-chipen_US
dc.subject.keywordoral fluiden_US
dc.subject.keywordmultiplexeden_US
dc.subject.keywordon-site testingen_US
dc.titleApplication of programmable bio-nano-chip system for the quantitative detection of drugs of abuse in oral fluidsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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