Crystal structure of SsfS6, the putative C-glycosyltransferase involved in SF2575 biosynthesis

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dc.citation.firstpage1277
dc.citation.issueNumber7
dc.citation.journalTitleProteins: Structure, Function, and Bioinformatics
dc.citation.lastpage1282
dc.citation.volumeNumber81
dc.contributor.authorWang, Fengbin
dc.contributor.authorZhou, Maoquan
dc.contributor.authorSingh, Shanteri
dc.contributor.authorYennamalli, Ragothaman M.
dc.contributor.authorBingman, Craig A.
dc.contributor.authorThorson, Jon S.
dc.contributor.authorPhillips, George N.Jr.
dc.date.accessioned2014-12-15T17:34:20Z
dc.date.available2014-12-15T17:34:20Z
dc.date.issued2013
dc.description.abstractThe molecule known as SF2575 from Streptomyces sp. is a tetracycline polyketide natural product that displays antitumor activity against murine leukemia P388 in vivo. In the SF2575 biosynthetic pathway, SsfS6 has been implicated as the crucial C-glycosyltransferase (C-GT) that forms the CC glycosidic bond between the sugar and the SF2575 tetracycline-like scaffold. Here, we report the crystal structure of SsfS6 in the free form and in complex with TDP, both at 2.4 Å resolution. The structures reveal SsfS6 to adopt a GT-B fold wherein the TDP and docked putative aglycon are consistent with the overall C-glycosylation reaction. As one of only a few existing structures for C-glycosyltransferases, the structures described herein may serve as a guide to better understand and engineer C-glycosylation.
dc.identifier.citationWang, Fengbin, Zhou, Maoquan, Singh, Shanteri, et al.. "Crystal structure of SsfS6, the putative C-glycosyltransferase involved in SF2575 biosynthesis." <i>Proteins: Structure, Function, and Bioinformatics,</i> 81, no. 7 (2013) Wiley: 1277-1282. http://dx.doi.org/10.1002/prot.24289.
dc.identifier.doihttp://dx.doi.org/10.1002/prot.24289
dc.identifier.urihttps://hdl.handle.net/1911/78740
dc.language.isoeng
dc.publisherWiley
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley.
dc.subject.keywordnatural product
dc.subject.keywordantitumor
dc.subject.keywordGT-B fold
dc.subject.keywordX-ray diffraction
dc.subject.keywordmolecular docking
dc.subject.keywordbiosynthesis
dc.subject.keywordcarbohydrate
dc.titleCrystal structure of SsfS6, the putative C-glycosyltransferase involved in SF2575 biosynthesis
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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