Microneedle-based sampling of dermal interstitial fluid using a vacuum-assisted skin patch

dc.citation.articleNumber101975en_US
dc.citation.issueNumber6en_US
dc.citation.journalTitleCell Reports Physical Scienceen_US
dc.citation.volumeNumber5en_US
dc.contributor.authorJiang, Xueen_US
dc.contributor.authorWilkirson, Elizabeth C.en_US
dc.contributor.authorBailey, Aaron O.en_US
dc.contributor.authorRussell, William K.en_US
dc.contributor.authorLillehoj, Peter B.en_US
dc.date.accessioned2024-08-22T15:28:47Zen_US
dc.date.available2024-08-22T15:28:47Zen_US
dc.date.issued2024en_US
dc.description.abstractInterstitial fluid (ISF) contains a wealth of biomolecules, yet it is underutilized for diagnostic testing due to a lack of rapid and simple techniques for collecting abundant amounts of fluid. Here, we report a simple and minimally invasive technique for rapidly sampling larger quantities of ISF from human skin. A microneedle array is used to generate micropores in skin from which ISF is extracted using a vacuum-assisted skin patch. Using this technique, an average of 20.8 μL of dermal ISF is collected in 25 min, which is an ∼6-fold improvement over existing sampling methods. Proteomic analysis of collected ISF reveals that it has nearly identical protein composition as blood, and >600 medically relevant biomarkers are identified. Toward this end, we demonstrate the detection of SARS-CoV-2 neutralizing antibodies in ISF collected from COVID-19 vaccinees using two commercial immunoassays, showcasing the utility of this technique for diagnostic testing.en_US
dc.identifier.citationJiang, X., Wilkirson, E. C., Bailey, A. O., Russell, W. K., & Lillehoj, P. B. (2024). Microneedle-based sampling of dermal interstitial fluid using a vacuum-assisted skin patch. Cell Reports Physical Science, 5(6). https://doi.org/10.1016/j.xcrp.2024.101975en_US
dc.identifier.digitalPIIS2666386424002352en_US
dc.identifier.doihttps://doi.org/10.1016/j.xcrp.2024.101975en_US
dc.identifier.urihttps://hdl.handle.net/1911/117686en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsExcept where otherwise noted, this work is licensed under a Creative Commons Attribution (CC BY) license.  Permission to reuse, publish, or reproduce the work beyond the terms of the license or beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleMicroneedle-based sampling of dermal interstitial fluid using a vacuum-assisted skin patchen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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