Oncogene Concatenated Enriched Amplicon Nanopore Sequencing for rapid, accurate, and affordable somatic mutation detection

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dc.citation.articleNumber227
dc.citation.journalTitleGenome Biology
dc.citation.volumeNumber22
dc.contributor.authorThirunavukarasu, Deepak
dc.contributor.authorCheng, Lauren Y.
dc.contributor.authorSong, Ping
dc.contributor.authorChen, Sherry X.
dc.contributor.authorBorad, Mitesh J.
dc.contributor.authorKwong, Lawrence
dc.contributor.authorJames, Phillip
dc.contributor.authorTurner, Daniel J.
dc.contributor.authorZhang, David Yu
dc.date.accessioned2021-09-21T15:37:38Z
dc.date.available2021-09-21T15:37:38Z
dc.date.issued2021
dc.description.abstractWe develop the Oncogene Concatenated Enriched Amplicon Nanopore Sequencing (OCEANS) method, in which variants with low variant allele frequency (VAFs) are amplified and subsequently concatenated for Nanopore Sequencing. OCEANS allows accurate detection of somatic mutations with VAF limits of detection between 0.05 and 1%. We construct 4 distinct multi-gene OCEANS panels targeting recurrent mutations in acute myeloid leukemia, melanoma, non-small- cell lung cancer, and hepatocellular carcinoma and validate them on clinical samples. By demonstrating detection of low VAF single nucleotide variant mutations using Nanopore Sequencing, OCEANS is poised to enable same-day clinical sequencing panels.
dc.identifier.citationThirunavukarasu, Deepak, Cheng, Lauren Y., Song, Ping, et al.. "Oncogene Concatenated Enriched Amplicon Nanopore Sequencing for rapid, accurate, and affordable somatic mutation detection." <i>Genome Biology,</i> 22, (2021) Springer: https://doi.org/10.1186/s13059-021-02449-1.
dc.identifier.digitalOncogeneConcatenatedEnrichedAm
dc.identifier.doihttps://doi.org/10.1186/s13059-021-02449-1
dc.identifier.urihttps://hdl.handle.net/1911/111369
dc.language.isoeng
dc.publisherSpringer
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dc.rights.urihttp://creativecommons.org/licenses/by/4.0/.
dc.titleOncogene Concatenated Enriched Amplicon Nanopore Sequencing for rapid, accurate, and affordable somatic mutation detection
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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