Pyoverdine-Dependent Virulence ofﾠPseudomonas aeruginosaﾠIsolates From Cystic Fibrosis Patients

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dc.citation.journalTitleFrontiers in Microbiology
dc.contributor.authorKang, Donghoon
dc.contributor.authorRevtovich, Alexey V.
dc.contributor.authorChen, Qingquan
dc.contributor.authorShah, Kush N.
dc.contributor.authorCannon, Carolyn L.
dc.contributor.authorKirienko, Natalia V.
dc.date.accessioned2019-10-25T16:54:18Z
dc.date.available2019-10-25T16:54:18Z
dc.date.issued2019
dc.description.abstractThe development of therapies that modulate or prevent pathogen virulence may be a key strategy for circumventing antimicrobial resistance. Toward that end, we examined the production of pyoverdine, a key virulence determinant, in ∼70 Pseudomonas aeruginosa isolates from pediatric cystic fibrosis patients. Pyoverdine production was heterogeneous and showed a clear correlation with pathogenicity in Caenorhabditis elegans and an acute murine pneumonia model. Examination showed pyoverdine accumulation in host tissues, including extrapharyngeal tissues of C. elegans and lung tissues of mice, where accumulation correlated with host death. Many of the isolates tested were resistant to multiple antimicrobials, so we assayed the ability of pyoverdine inhibitors to mitigate virulence and rescue pyoverdine-mediated host pathology. Representatives from three different classes of pyoverdine inhibitors (gallium, fluoropyrimidines, and LK11) significantly improved survival. Our findings highlight the utility of targeting virulence factors in general, and pyoverdine in particular, as a promising method to control bacterial pathogenesis as the utility of antimicrobials continues to diminish.
dc.identifier.citationKang, Donghoon, Revtovich, Alexey V., Chen, Qingquan, et al.. "Pyoverdine-Dependent Virulence ofﾠPseudomonas aeruginosaﾠIsolates From Cystic Fibrosis Patients." <i>Frontiers in Microbiology,</i> (2019) Frontiers: https://doi.org/10.3389/fmicb.2019.02048.
dc.identifier.doihttps://doi.org/10.3389/fmicb.2019.02048
dc.identifier.urihttps://hdl.handle.net/1911/107515
dc.language.isoeng
dc.publisherFrontiers
dc.rightsThis is an open-access article distributed under the terms of theﾠCreative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titlePyoverdine-Dependent Virulence ofﾠPseudomonas aeruginosaﾠIsolates From Cystic Fibrosis Patients
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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