Pyoverdine-Dependent Virulence ofﾠPseudomonas aeruginosaﾠIsolates From Cystic Fibrosis Patients

Simple item page
dc.citation.journalTitleFrontiers in Microbiologyen_US
dc.contributor.authorKang, Donghoonen_US
dc.contributor.authorRevtovich, Alexey V.en_US
dc.contributor.authorChen, Qingquanen_US
dc.contributor.authorShah, Kush N.en_US
dc.contributor.authorCannon, Carolyn L.en_US
dc.contributor.authorKirienko, Natalia V.en_US
dc.date.accessioned2019-10-25T16:54:18Zen_US
dc.date.available2019-10-25T16:54:18Zen_US
dc.date.issued2019en_US
dc.description.abstractThe development of therapies that modulate or prevent pathogen virulence may be a key strategy for circumventing antimicrobial resistance. Toward that end, we examined the production of pyoverdine, a key virulence determinant, in ∼70 Pseudomonas aeruginosa isolates from pediatric cystic fibrosis patients. Pyoverdine production was heterogeneous and showed a clear correlation with pathogenicity in Caenorhabditis elegans and an acute murine pneumonia model. Examination showed pyoverdine accumulation in host tissues, including extrapharyngeal tissues of C. elegans and lung tissues of mice, where accumulation correlated with host death. Many of the isolates tested were resistant to multiple antimicrobials, so we assayed the ability of pyoverdine inhibitors to mitigate virulence and rescue pyoverdine-mediated host pathology. Representatives from three different classes of pyoverdine inhibitors (gallium, fluoropyrimidines, and LK11) significantly improved survival. Our findings highlight the utility of targeting virulence factors in general, and pyoverdine in particular, as a promising method to control bacterial pathogenesis as the utility of antimicrobials continues to diminish.en_US
dc.identifier.citationKang, Donghoon, Revtovich, Alexey V., Chen, Qingquan, et al.. "Pyoverdine-Dependent Virulence ofﾠPseudomonas aeruginosaﾠIsolates From Cystic Fibrosis Patients." <i>Frontiers in Microbiology,</i> (2019) Frontiers: https://doi.org/10.3389/fmicb.2019.02048.en_US
dc.identifier.doihttps://doi.org/10.3389/fmicb.2019.02048en_US
dc.identifier.urihttps://hdl.handle.net/1911/107515en_US
dc.language.isoengen_US
dc.publisherFrontiersen_US
dc.rightsThis is an open-access article distributed under the terms of theﾠCreative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titlePyoverdine-Dependent Virulence ofﾠPseudomonas aeruginosaﾠIsolates From Cystic Fibrosis Patientsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
fmicb-10-02048.pdf
Size:
2.63 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Faculty Publications
BioSciences Publications