Toward development of a large field-of-view cancer screening patch (CASP) to detect cervical intraepithelial neoplasia

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dc.citation.firstpage6145en_US
dc.citation.issueNumber12en_US
dc.citation.journalTitleBiomedical Optics Expressen_US
dc.citation.lastpage6159en_US
dc.citation.volumeNumber10en_US
dc.contributor.authorGawedzinski, Johnen_US
dc.contributor.authorSchmeler, Kathleen M.en_US
dc.contributor.authorMilbourne, Andreaen_US
dc.contributor.authorRamalingam, Preethaen_US
dc.contributor.authorMoghaddam, Parnian A.en_US
dc.contributor.authorRichards-Kortum, Rebeccaen_US
dc.contributor.authorRichards-Kortum, Rebeccaen_US
dc.contributor.authorTkaczyk, Tomasz S.en_US
dc.contributor.authorTkaczyk, Tomasz S.en_US
dc.contributor.orgBioengineeringen_US
dc.contributor.orgElectrical and Computer Engineeringen_US
dc.date.accessioned2021-10-06T14:15:44Zen_US
dc.date.available2021-10-06T14:15:44Zen_US
dc.date.issued2019en_US
dc.description.abstractCervical cancers are primarily diagnosed via colposcopy, in which the tissue is visually assessed by a clinician for abnormalities, followed by directed biopsies and histologic analysis of excised tissue. Optical biopsy technologies offer a less invasive method of imaging such that subcellular features can be resolved without removing tissue. These techniques, however, are limited in field-of-view by the distal end of the probe. We present a prototype that incorporates a rigid, machinable waveguide that is in direct contact with a fluorescently-labeled sample paired with a scanning fluorescent microscope. The system is capable of imaging large areas of tissue without the need to re-position the tissue-probe interface. A mosaicing algorithm was developed to quantify scanning shifts and stitch neighboring frames together to increase the field-of-view. Our prototype can yield a maximum axial resolution of <5 µm for individual frames and can produce mosaiced images with a field-of-view greater than 15 mm x 15 mm without sacrificing resolution. We validated the system with a 1951 USAF resolution target, fluorescent in vitro standards, and a patient study where ex vivo conization samples of squamous cervical epithelium were imaged. The results of the patient study indicate that architectural features of subcellular components could be detected and differentiated between normal tissue and precancerous lesions.en_US
dc.identifier.citationGawedzinski, John, Schmeler, Kathleen M., Milbourne, Andrea, et al.. "Toward development of a large field-of-view cancer screening patch (CASP) to detect cervical intraepithelial neoplasia." <i>Biomedical Optics Express,</i> 10, no. 12 (2019) Optical Society of America: 6145-6159. https://doi.org/10.1364/BOE.10.006145.en_US
dc.identifier.digitalboe-10-12-6145en_US
dc.identifier.doihttps://doi.org/10.1364/BOE.10.006145en_US
dc.identifier.urihttps://hdl.handle.net/1911/111479en_US
dc.language.isoengen_US
dc.publisherOptical Society of Americaen_US
dc.rightsPublished under the terms of the OSA Open Access Publishing Agreementen_US
dc.rights.urihttps://www.osapublishing.org/library/license_v1.cfmen_US
dc.titleToward development of a large field-of-view cancer screening patch (CASP) to detect cervical intraepithelial neoplasiaen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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