Changes in Optical Properties of Plasmonic Nanoparticles in Cellular Environments are Modulated by Nanoparticle PEGylation and Serum Conditions

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dc.citation.articleNumber303en_US
dc.citation.journalTitleNanoscale Research Lettersen_US
dc.citation.volumeNumber11en_US
dc.contributor.authorChen, Allen L.en_US
dc.contributor.authorJackson, Meredith A.en_US
dc.contributor.authorLin, Adam Y.en_US
dc.contributor.authorFigueroa, Elizabeth R.en_US
dc.contributor.authorHu, Ying S.en_US
dc.contributor.authorEvans, Emily R.en_US
dc.contributor.authorAsthana, Vishwaratnen_US
dc.contributor.authorYoung, Joseph K.en_US
dc.contributor.authorDrezek, Rebekah A.en_US
dc.date.accessioned2017-05-04T19:43:55Zen_US
dc.date.available2017-05-04T19:43:55Zen_US
dc.date.issued2016en_US
dc.description.abstractWhen plasmonic nanoparticles (NPs) are internalized by cells and agglomerate within intracellular vesicles, their optical spectra can shift and broaden as a result of plasmonic coupling of NPs in close proximity to one another. For such optical changes to be accounted for in the design of plasmonic NPs for light-based biomedical applications, quantitative design relationships between designable factors and spectral shifts need to be established. Here we begin building such a framework by investigating how functionalization of gold NPs (AuNPs) with biocompatible poly(ethylene) glycol (PEG), and the serum conditions in which the NPs are introduced to cells impact the optical changes exhibited by NPs in a cellular context. Utilizing darkfield hyperspectral imaging, we find that PEGylation decreases the spectral shifting and spectral broadening experienced by 100 nm AuNPs following uptake by Sk-Br-3 cells, but up to a 33 ± 12 nm shift in the spectral peak wavelength can still occur. The serum protein-containing biological medium also modulates the spectral changes experienced by cell-exposed NPs through the formation of a protein corona on the surface of NPs that mediates NP interactions with cells: PEGylated AuNPs exposed to cells in serum-free conditions experience greater spectral shifts than in serum-containing environments. Moreover, increased concentrations of serum (10, 25, or 50 %) result in the formation of smaller intracellular NP clusters and correspondingly reduced spectral shifts after 5 and 10 h NP-cell exposure. However, after 24 h, NP cluster size and spectral shifts are comparable and become independent of serum concentration. By elucidating the impact of PEGylation and serum concentration on the spectral changes experienced by plasmonic NPs in cells, this study provides a foundation for the optical engineering of plasmonic NPs for use in biomedical environments.en_US
dc.identifier.citationChen, Allen L., Jackson, Meredith A., Lin, Adam Y., et al.. "Changes in Optical Properties of Plasmonic Nanoparticles in Cellular Environments are Modulated by Nanoparticle PEGylation and Serum Conditions." <i>Nanoscale Research Letters,</i> 11, (2016) Springer: http://dx.doi.org/10.1186/s11671-016-1524-4.en_US
dc.identifier.doihttp://dx.doi.org/10.1186/s11671-016-1524-4en_US
dc.identifier.urihttps://hdl.handle.net/1911/94184en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subject.keywordCellsen_US
dc.subject.keywordGold nanoparticlesen_US
dc.subject.keywordHyperspectral imagingen_US
dc.subject.keywordNano-bio interactionsen_US
dc.subject.keywordNanomedicineen_US
dc.subject.keywordPlasmonicsen_US
dc.subject.keywordPoly(ethylene glycol)en_US
dc.subject.keywordProtein coronaen_US
dc.subject.keywordSerumen_US
dc.subject.keywordSpectral shiftingen_US
dc.titleChanges in Optical Properties of Plasmonic Nanoparticles in Cellular Environments are Modulated by Nanoparticle PEGylation and Serum Conditionsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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