Convenient analysis of protein modification by chemical blotting with fluorogenic “click” reagents

dc.citation.firstpage2846en_US
dc.citation.journalTitleMolecular BioSystemsen_US
dc.citation.lastpage2849en_US
dc.citation.volumeNumber11en_US
dc.contributor.authorOhata, Junen_US
dc.contributor.authorFarrukh, Vohidoven_US
dc.contributor.authorBall, Zachary T.en_US
dc.date.accessioned2017-06-14T18:46:24Zen_US
dc.date.available2017-06-14T18:46:24Zen_US
dc.date.issued2015en_US
dc.description.abstractDirect visualization of bioorthogonal alkyne or azide handles using fluorogenic azide–alkyne cycloaddition conducted on the surface of a blot membrane. The method eliminates the need for separation steps to remove excess small molecule reagents before attachment of antigen molecules or other visualization handles, and is especially useful for the analysis of peptides and small proteins. A variety of potential fluorogenic reagents are assessed, and sensitivity (<0.1 picomole) similar to current commercially available fluorescence imaging methods is possible.en_US
dc.identifier.citationOhata, Jun, Farrukh, Vohidov and Ball, Zachary T.. "Convenient analysis of protein modification by chemical blotting with fluorogenic “click” reagents." <i>Molecular BioSystems,</i> 11, (2015) Royal Society of Chemistry: 2846-2849. https://doi.org/10.1039/C5MB00510H.en_US
dc.identifier.doihttps://doi.org/10.1039/C5MB00510Hen_US
dc.identifier.urihttps://hdl.handle.net/1911/94851en_US
dc.language.isoengen_US
dc.publisherRoyal Society of Chemistryen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the Royal Society of Chemistry.en_US
dc.titleConvenient analysis of protein modification by chemical blotting with fluorogenic “click” reagentsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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