A high-throughput three-dimensional cell migration assay for toxicity screening with mobile device-based macroscopic image analysis

Simple item page
dc.citation.journalTitleScientific Reportsen_US
dc.citation.volumeNumber3en_US
dc.contributor.authorTimm, David M.en_US
dc.contributor.authorChen, Jianboen_US
dc.contributor.authorSing, Daviden_US
dc.contributor.authorGage, Jacob A.en_US
dc.contributor.authorHaisler, William L.en_US
dc.contributor.authorNeeley, Shane K.en_US
dc.contributor.authorRaphael, Robert M.en_US
dc.contributor.authorDehghani, Mehdien_US
dc.contributor.authorRosenblatt, Kevin P.en_US
dc.contributor.authorKillian, T.C.en_US
dc.contributor.authorTseng, Huberten_US
dc.contributor.authorSouza, Glauco R.en_US
dc.date.accessioned2013-12-20T21:08:48Zen_US
dc.date.available2013-12-20T21:08:48Zen_US
dc.date.issued2013en_US
dc.description.abstractThere is a growing demand for in vitro assays for toxicity screening in three-dimensional (3D) environments. In this study, 3D cell culture using magnetic levitation was used to create an assay in which cells were patterned into 3D rings that close over time. The rate of closure was determined from time-lapse images taken with a mobile device and related to drug concentration. Rings of human embryonic kidney cells (HEK293) and tracheal smooth muscle cells (SMCs) were tested with ibuprofen and sodium dodecyl sulfate (SDS). Ring closure correlated with the viability and migration of cells in two dimensions (2D). Images taken using a mobile device were similar in analysis to images taken with a microscope. Ring closure may serve as a promising label-free and quantitative assay for high-throughput in vivo toxicity in 3D cultures.en_US
dc.identifier.citationTimm, David M., Chen, Jianbo, Sing, David, et al.. "A high-throughput three-dimensional cell migration assay for toxicity screening with mobile device-based macroscopic image analysis." <i>Scientific Reports,</i> 3, (2013) Nature Publishing Group: http://dx.doi.org/10.1038/srep03000.en_US
dc.identifier.doihttp://dx.doi.org/10.1038/srep03000en_US
dc.identifier.urihttps://hdl.handle.net/1911/75309en_US
dc.language.isoengen_US
dc.publisherNature Publishing Groupen_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.subject.keywordbiological modelsen_US
dc.subject.keywordtoxicologyen_US
dc.subject.keywordcell migrationen_US
dc.subject.keywordassay systemsen_US
dc.titleA high-throughput three-dimensional cell migration assay for toxicity screening with mobile device-based macroscopic image analysisen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
high-throughput.pdf
Size:
4.03 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Bioengineering Publications
Faculty Publications
Physics and Astronomy Publications