A high-throughput three-dimensional cell migration assay for toxicity screening with mobile device-based macroscopic image analysis

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dc.citation.journalTitleScientific Reports
dc.citation.volumeNumber3
dc.contributor.authorTimm, David M.
dc.contributor.authorChen, Jianbo
dc.contributor.authorSing, David
dc.contributor.authorGage, Jacob A.
dc.contributor.authorHaisler, William L.
dc.contributor.authorNeeley, Shane K.
dc.contributor.authorRaphael, Robert M.
dc.contributor.authorDehghani, Mehdi
dc.contributor.authorRosenblatt, Kevin P.
dc.contributor.authorKillian, T.C.
dc.contributor.authorTseng, Hubert
dc.contributor.authorSouza, Glauco R.
dc.date.accessioned2013-12-20T21:08:48Z
dc.date.available2013-12-20T21:08:48Z
dc.date.issued2013
dc.description.abstractThere is a growing demand for in vitro assays for toxicity screening in three-dimensional (3D) environments. In this study, 3D cell culture using magnetic levitation was used to create an assay in which cells were patterned into 3D rings that close over time. The rate of closure was determined from time-lapse images taken with a mobile device and related to drug concentration. Rings of human embryonic kidney cells (HEK293) and tracheal smooth muscle cells (SMCs) were tested with ibuprofen and sodium dodecyl sulfate (SDS). Ring closure correlated with the viability and migration of cells in two dimensions (2D). Images taken using a mobile device were similar in analysis to images taken with a microscope. Ring closure may serve as a promising label-free and quantitative assay for high-throughput in vivo toxicity in 3D cultures.
dc.identifier.citationTimm, David M., Chen, Jianbo, Sing, David, et al.. "A high-throughput three-dimensional cell migration assay for toxicity screening with mobile device-based macroscopic image analysis." <i>Scientific Reports,</i> 3, (2013) Nature Publishing Group: http://dx.doi.org/10.1038/srep03000.
dc.identifier.doihttp://dx.doi.org/10.1038/srep03000
dc.identifier.urihttps://hdl.handle.net/1911/75309
dc.language.isoeng
dc.publisherNature Publishing Group
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
dc.subject.keywordbiological models
dc.subject.keywordtoxicology
dc.subject.keywordcell migration
dc.subject.keywordassay systems
dc.titleA high-throughput three-dimensional cell migration assay for toxicity screening with mobile device-based macroscopic image analysis
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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