A protocol for whole-mount immuno-coupled hybridization chain reaction (WICHCR) in zebrafish embryos and larvae

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dc.citation.articleNumber100709en_US
dc.citation.issueNumber3en_US
dc.citation.journalTitleSTAR Protocolsen_US
dc.citation.volumeNumber2en_US
dc.contributor.authorIbarra-García-Padilla, Rodrigoen_US
dc.contributor.authorHoward, Aubrey Gaylon Adamen_US
dc.contributor.authorSingleton, Eileen Willeyen_US
dc.contributor.authorUribe, Rosa Annaen_US
dc.date.accessioned2021-08-20T20:24:26Zen_US
dc.date.available2021-08-20T20:24:26Zen_US
dc.date.issued2021en_US
dc.description.abstractCharacterizing mRNA and protein expression with temporal and spatial resolution is a valuable component of nearly every developmental study. Here, we describe a protocol that combines in situ hybridization chain reaction (HCR) and immunofluorescence, allowing for the detection of mRNAs and proteins simultaneously, in zebrafish embryos and larvae. This protocol expands the flexibility of multiplexed HCR by coupling it with traditional immunofluorescence detection. For complete details on the use and execution of this protocol, please refer to Choi et al. (2010, 2016, 2018) and Howard et al. (2021).en_US
dc.identifier.citationIbarra-García-Padilla, Rodrigo, Howard, Aubrey Gaylon Adam, Singleton, Eileen Willey, et al.. "A protocol for whole-mount immuno-coupled hybridization chain reaction (WICHCR) in zebrafish embryos and larvae." <i>STAR Protocols,</i> 2, no. 3 (2021) Cell Press: https://doi.org/10.1016/j.xpro.2021.100709.en_US
dc.identifier.digital1-s2-0-S2666166721004160-mainen_US
dc.identifier.doihttps://doi.org/10.1016/j.xpro.2021.100709en_US
dc.identifier.urihttps://hdl.handle.net/1911/111309en_US
dc.language.isoengen_US
dc.publisherCell Pressen_US
dc.rightsThis is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/).en_US
dc.titleA protocol for whole-mount immuno-coupled hybridization chain reaction (WICHCR) in zebrafish embryos and larvaeen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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