Sustained delivery of recombinant human bone morphogenetic protein-2 from perlecan domain I - functionalized electrospun poly (ε-caprolactone) scaffolds for bone regeneration

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dc.citation.articleNumber25
dc.citation.journalTitleJournal of Experimental Orthopaedics
dc.citation.volumeNumber3
dc.contributor.authorChiu, Yu-Chieh
dc.contributor.authorFong, Eliza L.S.
dc.contributor.authorGrindel, Brian J.
dc.contributor.authorKasper, Fred K.
dc.contributor.authorHarrington, Daniel Anton
dc.contributor.authorFarach-Carson, Mary C.
dc.date.accessioned2017-03-07T17:07:15Z
dc.date.available2017-03-07T17:07:15Z
dc.date.issued2016
dc.description.abstractBackground: Biomaterial scaffolds that deliver growth factors such as recombinant human bone morphogenetic proteins-2 (rhBMP-2) have improved clinical bone tissue engineering by enhancing bone tissue regeneration. This approach could be further improved if the controlled delivery of bioactive rhBMP-2 were sustained throughout the duration of osteogenesis from fibrous scaffolds that provide control over dose and bioactivity of rhBMP-2. In nature, heparan sulfate attached to core proteoglycans serves as the co-receptor that delivers growth factors to support tissue morphogenesis. Methods: To mimic this behavior, we conjugated heparan sulfate decorated recombinant domain I of perlecan/HSPG2 onto an electrospun poly(ε-caprolactone) (PCL) scaffold, hypothesizing that the heparan sulfate chains will enhance rhBMP-2 loading onto the scaffold and preserve delivered rhBMP-2 bioactivity. Results: In this study, we demonstrated that covalently conjugated perlecan domain I increased loading capacity of rhBMP-2 onto PCL scaffolds when compared to control unconjugated scaffolds. Additionally, rhBMP-2 released from the modified scaffolds enhanced alkaline phosphatase activity in W20–17 mouse bone marrow stromal cells, indicating the preservation of rhBMP-2 bioactivity indicative of osteogenesis. Conclusions: We conclude that this platform provides a sophisticated and efficient approach to deliver bioactive rhBMP-2 for bone tissue regeneration applications.
dc.identifier.citationChiu, Yu-Chieh, Fong, Eliza L.S., Grindel, Brian J., et al.. "Sustained delivery of recombinant human bone morphogenetic protein-2 from perlecan domain I - functionalized electrospun poly (ε-caprolactone) scaffolds for bone regeneration." <i>Journal of Experimental Orthopaedics,</i> 3, (2016) Springer Nature: http://dx.doi.org/10.1186/s40634-016-0057-1.
dc.identifier.doihttp://dx.doi.org/10.1186/s40634-016-0057-1
dc.identifier.urihttps://hdl.handle.net/1911/94022
dc.language.isoeng
dc.publisherSpringer Nature
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.keywordHeparan sulfate
dc.subject.keywordPoly(ε-caprolactone)
dc.subject.keywordBone morphogenetic protein
dc.subject.keywordAlkaline phosphatase
dc.subject.keywordPerlecan/HSPG2
dc.subject.keywordBone regeneration
dc.titleSustained delivery of recombinant human bone morphogenetic protein-2 from perlecan domain I - functionalized electrospun poly (ε-caprolactone) scaffolds for bone regeneration
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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