Widefield imaging of rapid pan-cortical voltage dynamics with an indicator evolved for one-photon microscopy

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dc.citation.articleNumber6423
dc.citation.journalTitleNature Communications
dc.citation.volumeNumber14
dc.contributor.authorLu, Xiaoyu
dc.contributor.authorWang, Yunmiao
dc.contributor.authorLiu, Zhuohe
dc.contributor.authorGou, Yueyang
dc.contributor.authorJaeger, Dieter
dc.contributor.authorSt-Pierre, François
dc.date.accessioned2024-05-03T15:51:18Z
dc.date.available2024-05-03T15:51:18Z
dc.date.issued2023
dc.description.abstractWidefield imaging with genetically encoded voltage indicators (GEVIs) is a promising approach for understanding the role of large cortical networks in the neural coding of behavior. However, the limited performance of current GEVIs restricts their deployment for single-trial imaging of rapid neuronal voltage dynamics. Here, we developed a high-throughput platform to screen for GEVIs that combine fast kinetics with high brightness, sensitivity, and photostability under widefield one-photon illumination. Rounds of directed evolution produced JEDI-1P, a green-emitting fluorescent indicator with enhanced performance across all metrics. Next, we optimized a neonatal intracerebroventricular delivery method to achieve cost-effective and wide-spread JEDI-1P expression in mice. We also developed an approach to correct optical measurements from hemodynamic and motion artifacts effectively. Finally, we achieved stable brain-wide voltage imaging and successfully tracked gamma-frequency whisker and visual stimulations in awake mice in single trials, opening the door to investigating the role of high-frequency signals in brain computations.
dc.identifier.citationLu, X., Wang, Y., Liu, Z., Gou, Y., Jaeger, D., & St-Pierre, F. (2023). Widefield imaging of rapid pan-cortical voltage dynamics with an indicator evolved for one-photon microscopy. Nature Communications, 14(1), 6423. https://doi.org/10.1038/s41467-023-41975-3
dc.identifier.digitals41467-023-41975-3
dc.identifier.doihttps://doi.org/10.1038/s41467-023-41975-3
dc.identifier.urihttps://hdl.handle.net/1911/115612
dc.language.isoeng
dc.publisherSpringer Nature
dc.rightsExcept where otherwise noted, this work is licensed under a Creative Commons Attribution (CC BY) license. Permission to reuse, publish, or reproduce the work beyond the terms of the license or beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleWidefield imaging of rapid pan-cortical voltage dynamics with an indicator evolved for one-photon microscopy
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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