Widefield imaging of rapid pan-cortical voltage dynamics with an indicator evolved for one-photon microscopy

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dc.citation.articleNumber6423en_US
dc.citation.journalTitleNature Communicationsen_US
dc.citation.volumeNumber14en_US
dc.contributor.authorLu, Xiaoyuen_US
dc.contributor.authorWang, Yunmiaoen_US
dc.contributor.authorLiu, Zhuoheen_US
dc.contributor.authorGou, Yueyangen_US
dc.contributor.authorJaeger, Dieteren_US
dc.contributor.authorSt-Pierre, Françoisen_US
dc.date.accessioned2024-05-03T15:51:18Zen_US
dc.date.available2024-05-03T15:51:18Zen_US
dc.date.issued2023en_US
dc.description.abstractWidefield imaging with genetically encoded voltage indicators (GEVIs) is a promising approach for understanding the role of large cortical networks in the neural coding of behavior. However, the limited performance of current GEVIs restricts their deployment for single-trial imaging of rapid neuronal voltage dynamics. Here, we developed a high-throughput platform to screen for GEVIs that combine fast kinetics with high brightness, sensitivity, and photostability under widefield one-photon illumination. Rounds of directed evolution produced JEDI-1P, a green-emitting fluorescent indicator with enhanced performance across all metrics. Next, we optimized a neonatal intracerebroventricular delivery method to achieve cost-effective and wide-spread JEDI-1P expression in mice. We also developed an approach to correct optical measurements from hemodynamic and motion artifacts effectively. Finally, we achieved stable brain-wide voltage imaging and successfully tracked gamma-frequency whisker and visual stimulations in awake mice in single trials, opening the door to investigating the role of high-frequency signals in brain computations.en_US
dc.identifier.citationLu, X., Wang, Y., Liu, Z., Gou, Y., Jaeger, D., & St-Pierre, F. (2023). Widefield imaging of rapid pan-cortical voltage dynamics with an indicator evolved for one-photon microscopy. Nature Communications, 14(1), 6423. https://doi.org/10.1038/s41467-023-41975-3en_US
dc.identifier.digitals41467-023-41975-3en_US
dc.identifier.doihttps://doi.org/10.1038/s41467-023-41975-3en_US
dc.identifier.urihttps://hdl.handle.net/1911/115612en_US
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.rightsExcept where otherwise noted, this work is licensed under a Creative Commons Attribution (CC BY) license. Permission to reuse, publish, or reproduce the work beyond the terms of the license or beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleWidefield imaging of rapid pan-cortical voltage dynamics with an indicator evolved for one-photon microscopyen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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