Gold Nanoparticle Aggregation for Quantification of Oligonucleotides: Optimization and Increased Dynamic Range

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dc.citation.firstpage99
dc.citation.issueNumber2
dc.citation.journalTitleAnalytical Biochemistry
dc.citation.lastpage105
dc.citation.volumeNumber431
dc.contributor.authorCordray, Michael S.
dc.contributor.authorAmdahl, Matthew
dc.contributor.authorRichards-Kortum, Rebecca R.
dc.date.accessioned2014-10-29T18:15:02Z
dc.date.available2014-10-29T18:15:02Z
dc.date.issued2012
dc.description.abstractA variety of assays have been proposed to detect small quantities of nucleic acids at the point-of-care. One approach relies on target-induced aggregation of gold nanoparticles functionalized with oligonucleotide sequences complementary to adjacent regions on the targeted sequence. In the presence of the target sequence, the gold nanoparticles aggregate, producing an easily detectable shift in the optical scattering properties of the solution. The major limitations of this assay are that it requires heating, and that long incubation times are required to produce a result. This study aims to optimize the assay conditions and optical readout, with the goals of eliminating the need for heating and reducing the time to result without sacrificing sensitivity or dynamic range. By optimizing assay conditions and measuring the spectrum of scattered light at the endpoint of incubation, we find that the assay is capable of producing quantifiable results at room temperature in 30 minutes with a linear dynamic range spanning from 150 amoles to 15 fmoles of target. If changes in light scattering are measured dynamically during the incubation process, the linear range can be expanded 2-fold, spanning 50 amoles to 500 fmoles, while decreasing the time to result down to 10 minutes.
dc.identifier.citationCordray, Michael S., Amdahl, Matthew and Richards-Kortum, Rebecca R.. "Gold Nanoparticle Aggregation for Quantification of Oligonucleotides: Optimization and Increased Dynamic Range." <i>Analytical Biochemistry,</i> 431, no. 2 (2012) Elsevier: 99-105. http://dx.doi.org/10.1016/j.ab.2012.09.013.
dc.identifier.doihttp://dx.doi.org/10.1016/j.ab.2012.09.013
dc.identifier.urihttps://hdl.handle.net/1911/77666
dc.language.isoeng
dc.publisherElsevier
dc.rightsOpen access under CC BY-NC-ND license .
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.keywordgold nanoparticles
dc.subject.keywordoligonucleotides
dc.subject.keywordoptical scattering
dc.subject.keywordquantification
dc.titleGold Nanoparticle Aggregation for Quantification of Oligonucleotides: Optimization and Increased Dynamic Range
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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