Gentamicin Reduces Calcific Nodule Formation by Aortic Valve Interstitial Cells In Vitro

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dc.citation.firstpage16en_US
dc.citation.issueNumber1en_US
dc.citation.journalTitleCardiovascular Engineering and Technologyen_US
dc.citation.lastpage25en_US
dc.citation.volumeNumber4en_US
dc.contributor.authorKumar, Adityaen_US
dc.contributor.authorWiltz, Dena C.en_US
dc.contributor.authorGrande-Allen, K. Janeen_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2014-12-15T20:35:09Zen_US
dc.date.available2014-12-15T20:35:09Zen_US
dc.date.issued2013en_US
dc.description.abstractGentamicin is a widely employed antibiotic, but may reduce calcium uptake by eukaryotic cells. This study was conducted to determine whether gentamicin reduces calcification by porcine aortic valvular interstitial cells (pAVICs) grown in 2D culture, which is a common model for calcific aortic valve disease (CAVD). The presence of gentamicin (up to 0.2 mM) in the medium of pAVICs cultured for 8 days significantly lowered calcification and alkaline phosphatase content in a dose-dependent manner compared to pAVICs cultured without gentamicin. Gentamicin also significantly increased cell proliferation and apoptosis at concentrations of 0.1–0.2 mM compared to controls. Next, gentamicin was applied to previously calcified pAVIC cultures (grown for 8 days) to determine whether it could stop or reverse the calcification process. Daily application of gentamicin for 8 additional days significantly reduced calcification to below the pre-calcification levels. These results confirm that gentamicin should be used cautiously with in vitro studies of calcification, and suggest that gentamicin may have the ability to reverse calcification by pAVICs. Given the nephrotoxicity and ototoxicity of this antibiotic, its clinical potential for the treatment of calcification in heart valves is limited. However, further investigation of the pathways through which gentamicin alters calcium uptake by valvular cells may provide insight into novel therapies for CAVD.en_US
dc.identifier.citationKumar, Aditya, Wiltz, Dena C. and Grande-Allen, K. Jane. "Gentamicin Reduces Calcific Nodule Formation by Aortic Valve Interstitial Cells In Vitro." <i>Cardiovascular Engineering and Technology,</i> 4, no. 1 (2013) Springer: 16-25. http://dx.doi.org/10.1007/s13239-012-0114-6.en_US
dc.identifier.doihttp://dx.doi.org/10.1007/s13239-012-0114-6en_US
dc.identifier.urihttps://hdl.handle.net/1911/78757en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Springer.en_US
dc.subject.keywordgentamicinen_US
dc.subject.keywordcalcificationen_US
dc.subject.keywordmitochondrial calcium uptakeen_US
dc.subject.keywordcell cultureen_US
dc.titleGentamicin Reduces Calcific Nodule Formation by Aortic Valve Interstitial Cells In Vitroen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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