Gentamicin Reduces Calcific Nodule Formation by Aortic Valve Interstitial Cells In Vitro

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dc.citation.firstpage16
dc.citation.issueNumber1
dc.citation.journalTitleCardiovascular Engineering and Technology
dc.citation.lastpage25
dc.citation.volumeNumber4
dc.contributor.authorKumar, Aditya
dc.contributor.authorWiltz, Dena C.
dc.contributor.authorGrande-Allen, K. Jane
dc.date.accessioned2014-12-15T20:35:09Z
dc.date.available2014-12-15T20:35:09Z
dc.date.issued2013
dc.description.abstractGentamicin is a widely employed antibiotic, but may reduce calcium uptake by eukaryotic cells. This study was conducted to determine whether gentamicin reduces calcification by porcine aortic valvular interstitial cells (pAVICs) grown in 2D culture, which is a common model for calcific aortic valve disease (CAVD). The presence of gentamicin (up to 0.2 mM) in the medium of pAVICs cultured for 8 days significantly lowered calcification and alkaline phosphatase content in a dose-dependent manner compared to pAVICs cultured without gentamicin. Gentamicin also significantly increased cell proliferation and apoptosis at concentrations of 0.1–0.2 mM compared to controls. Next, gentamicin was applied to previously calcified pAVIC cultures (grown for 8 days) to determine whether it could stop or reverse the calcification process. Daily application of gentamicin for 8 additional days significantly reduced calcification to below the pre-calcification levels. These results confirm that gentamicin should be used cautiously with in vitro studies of calcification, and suggest that gentamicin may have the ability to reverse calcification by pAVICs. Given the nephrotoxicity and ototoxicity of this antibiotic, its clinical potential for the treatment of calcification in heart valves is limited. However, further investigation of the pathways through which gentamicin alters calcium uptake by valvular cells may provide insight into novel therapies for CAVD.
dc.identifier.citationKumar, Aditya, Wiltz, Dena C. and Grande-Allen, K. Jane. "Gentamicin Reduces Calcific Nodule Formation by Aortic Valve Interstitial Cells In Vitro." <i>Cardiovascular Engineering and Technology,</i> 4, no. 1 (2013) Springer: 16-25. http://dx.doi.org/10.1007/s13239-012-0114-6.
dc.identifier.doihttp://dx.doi.org/10.1007/s13239-012-0114-6
dc.identifier.urihttps://hdl.handle.net/1911/78757
dc.language.isoeng
dc.publisherSpringer
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Springer.
dc.subject.keywordgentamicin
dc.subject.keywordcalcification
dc.subject.keywordmitochondrial calcium uptake
dc.subject.keywordcell culture
dc.titleGentamicin Reduces Calcific Nodule Formation by Aortic Valve Interstitial Cells In Vitro
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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