Redistribution of intracellular and extracellular free & adsorbed antibiotic resistance genes through a wastewater treatment plant by an enhanced extracellular DNA extraction method with magnetic beads

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dc.citation.articleNumber104986en_US
dc.citation.journalTitleEnvironment Internationalen_US
dc.citation.volumeNumber131en_US
dc.contributor.authorYuan, Qing-Binen_US
dc.contributor.authorHuang, Ya-Mengen_US
dc.contributor.authorWu, Wen-Binen_US
dc.contributor.authorZuo, Pengxiaoen_US
dc.contributor.authorHu, Nanen_US
dc.contributor.authorZhou, Yong-Zhangen_US
dc.contributor.authorAlvarez, Pedro J.J.en_US
dc.date.accessioned2019-08-28T16:10:12Zen_US
dc.date.available2019-08-28T16:10:12Zen_US
dc.date.issued2019en_US
dc.description.abstractDue to the limitations of current extraction methods, extracellular DNA (eDNA) is rarely discerned from intracellular DNA (iDNA) despite having unique contributions to antibiotic resistance genes (ARGs) propagation. Furthermore, eDNA may be free (f-eDNA) or adsorbed to or suspended solids, including cells (a-eDNA), which affects ARG persistence and transmissivity. We developed a novel method using magnetic beads to separate iDNA, a-eDNA, and f-eDNA to assess how these physical states of ARGs change across a wastewater treatment plant. This method efficiently extracted eDNA (>85.3%) with higher recovery than current methods such as alcohol precipitation, CTAB-based extraction, and DNA extraction kits (<10%). Biological treatment and UV disinfection decreased the concentration of intracellular ARGs (iARGs) and adsorbed extracellular ARGs (a-eARGs), causing an increase of released free extracellular ARGs (f-eARGs). More ARGs were discharged through the wasted biosolids than in the effluent; iARGs and a-eARGs are prevalent in wasted biosolids ((73.9 ± 22.5) % and (23.4 ± 15.3) % of total ARGs respectively), while f-eARGs were prevalent in the effluent ((90.3 ± 16.5) %). Bacterial community analysis showed significant correlations between specific genera and ARGs (e.g., Aeromonas, Pseudomonas and Acinetobacter were strongly correlated with multidrug-resistance gene blaTEM). This treatment system decreased the discharge of iARGs to receiving environments, however, increased eARG concentrations were present in the effluent, which may contribute to the environmental resistome.en_US
dc.identifier.citationYuan, Qing-Bin, Huang, Ya-Meng, Wu, Wen-Bin, et al.. "Redistribution of intracellular and extracellular free & adsorbed antibiotic resistance genes through a wastewater treatment plant by an enhanced extracellular DNA extraction method with magnetic beads." <i>Environment International,</i> 131, (2019) Elsevier: https://doi.org/10.1016/j.envint.2019.104986.en_US
dc.identifier.doihttps://doi.org/10.1016/j.envint.2019.104986en_US
dc.identifier.urihttps://hdl.handle.net/1911/107372en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsThis is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).en_US
dc.rights.urihttp://creativecommons.org/licenses/BY-NC-ND/4.0/en_US
dc.subject.keywordAntibiotic resistance genesen_US
dc.subject.keywordBacterial communityen_US
dc.subject.keywordDNA extractionen_US
dc.subject.keywordExtracellular DNAen_US
dc.subject.keywordMagnetic beadsen_US
dc.subject.keywordWastewater treatment facilityen_US
dc.titleRedistribution of intracellular and extracellular free & adsorbed antibiotic resistance genes through a wastewater treatment plant by an enhanced extracellular DNA extraction method with magnetic beadsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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