Rapid Metabolism of 1,4-Dioxane to below Health Advisory Levels by Thiamine-Amended Rhodococcus ruber Strain 219

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dc.citation.firstpage975en_US
dc.citation.issueNumber11en_US
dc.citation.journalTitleEnvironmental Science & Technology Lettersen_US
dc.citation.lastpage980en_US
dc.citation.volumeNumber8en_US
dc.contributor.authorSimmer, Reid A.en_US
dc.contributor.authorRichards, Patrick M.en_US
dc.contributor.authorEwald, Jessica M.en_US
dc.contributor.authorSchwarz, Coryen_US
dc.contributor.authorda Silva, Marcio L.B.en_US
dc.contributor.authorMathieu, Jacquesen_US
dc.contributor.authorAlvarez, Pedro J.J.en_US
dc.contributor.authorSchnoor, Jerald L.en_US
dc.date.accessioned2021-12-02T15:09:21Zen_US
dc.date.available2021-12-02T15:09:21Zen_US
dc.date.issued2021en_US
dc.description.abstractBioremediation is a promising treatment technology for 1,4-dioxane-contaminated groundwater. However, metabolic dioxane-degrading bacteria identified to date are limited by their slow kinetics and inability to sustain growth at low dioxane concentrations (<100 μg/L). Furthermore, strains may underperform because of missing growth factors, such as amino acids or vitamins. In this work, we reevaluate Rhodococcus ruber strain 219 as a dioxane-degrading strain with bioaugmentation potential. We report rapid growth and metabolic dioxane degradation by R. ruber 219 when supplemented with thiamine (vitamin B1). We also discern that the strain lacks a complete de novo thiamine synthesis pathway, indicating that R. ruber 219 is a probable thiamine auxotroph. However, when supplemented with thiamine, the strain’s Monod kinetics (Ks = 0.015 ± 0.03 μg/L) and exceedingly low Smin (0.49 ± 1.16 μg/L) suggest this strain can maintain growth at very low dioxane concentrations (<100 μg/L). Accordingly, we demonstrate that thiamine-grown R. ruber 219 sustains degradation of dilute dioxane (<100 μg/L) to below health advisory levels. This is the first study to report sustained metabolic dioxane biodegradation to below health advisory levels of 0.35 μg/L. Overall, our findings solidify R. ruber 219 as a promising candidate for bioremediation of dioxane-contaminated groundwater.en_US
dc.identifier.citationSimmer, Reid A., Richards, Patrick M., Ewald, Jessica M., et al.. "Rapid Metabolism of 1,4-Dioxane to below Health Advisory Levels by Thiamine-Amended Rhodococcus ruber Strain 219." <i>Environmental Science & Technology Letters,</i> 8, no. 11 (2021) American Chemical Society: 975-980. https://doi.org/10.1021/acs.estlett.1c00714.en_US
dc.identifier.digitalacs-estlett-1c00714en_US
dc.identifier.doihttps://doi.org/10.1021/acs.estlett.1c00714en_US
dc.identifier.urihttps://hdl.handle.net/1911/111720en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution Licenseen_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleRapid Metabolism of 1,4-Dioxane to below Health Advisory Levels by Thiamine-Amended Rhodococcus ruber Strain 219en_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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