Measuring Competitive Fitness in Dynamic Environments

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dc.citation.firstpage13175en_US
dc.citation.issueNumber42en_US
dc.citation.journalTitleThe Journal of Physical Chemistry Ben_US
dc.citation.lastpage13181en_US
dc.citation.volumeNumber117en_US
dc.contributor.authorRazinkov, Ivan A.en_US
dc.contributor.authorBaumgartner, Bridget L.en_US
dc.contributor.authorBennett, Matthew R.en_US
dc.contributor.authorTsimring, Lev S.en_US
dc.contributor.authorHasty, Jeffen_US
dc.contributor.orgInstitute of Biosciences and Bioengineeringen_US
dc.date.accessioned2015-07-09T20:14:49Zen_US
dc.date.available2015-07-09T20:14:49Zen_US
dc.date.issued2013en_US
dc.description.abstractMost yeast genes are dispensable for optimal growth in laboratory cultures. However, this apparent lack of fitness contribution is difficult to reconcile with the theory of natural selection. Here we use stochastic modeling to show that environmental fluctuations can select for a genetic mechanism that does not affect growth in static laboratory environments. We then present a novel experimental platform for measuring the fitness levels of specific genotypes in fluctuating environments. We test this platform by monitoring a mixed culture of two yeast strains that differ in their ability to respond to changes in carbon source yet exhibit the same fitness level in static conditions. When the sugar in the growth medium was switched between galactose and glucose, the wild-type strain gained a growth advantage over the mutant strain. Interestingly, both our computational and experimental results show that the strength of the adaptive advantage conveyed by the wild-type genotype depends on the total number of carbon source switches, not on the frequency of these fluctuations. Our results illustrate the selective power of environmental fluctuations on seemingly slight phenotypic differences in cellular response dynamics and underscore the importance of dynamic processes in the evolution of species.en_US
dc.identifier.citationRazinkov, Ivan A., Baumgartner, Bridget L., Bennett, Matthew R., et al.. "Measuring Competitive Fitness in Dynamic Environments." <i>The Journal of Physical Chemistry B,</i> 117, no. 42 (2013) American Chemical Society: 13175-13181. http://dx.doi.org/10.1021/jp403162v.en_US
dc.identifier.doihttp://dx.doi.org/10.1021/jp403162ven_US
dc.identifier.urihttps://hdl.handle.net/1911/80869en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Chemical Society.en_US
dc.subject.keywordevolutionen_US
dc.subject.keywordmicrofluidicsen_US
dc.subject.keywordmetabolic regulationen_US
dc.subject.keywordcompetitionen_US
dc.titleMeasuring Competitive Fitness in Dynamic Environmentsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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