Programmable bio-nanochip-based cytologic testing of oral potentially malignant disorders in Fanconi anemia

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dc.citation.firstpage593en_US
dc.citation.issueNumber5en_US
dc.citation.journalTitleOral Diseasesen_US
dc.citation.lastpage601en_US
dc.citation.volumeNumber21en_US
dc.contributor.authorFloriano, Pierreen_US
dc.contributor.authorAbram, Timen_US
dc.contributor.authorTaylor, Leanderen_US
dc.contributor.authorLe, Cathyen_US
dc.contributor.authorTalavera, Humbertoen_US
dc.contributor.authorNguyen, Michaelen_US
dc.contributor.authorRaja, Rameezen_US
dc.contributor.authorGillenwater, Annen_US
dc.contributor.authorMcDevitt, Johnen_US
dc.contributor.authorVigneswaran, Nadarajahen_US
dc.date.accessioned2017-06-14T16:55:02Zen_US
dc.date.available2017-06-14T16:55:02Zen_US
dc.date.issued2015en_US
dc.description.abstractFanconi anemia (FA) is caused by mutations of DNA repair genes. The risk of oral squamous cell carcinoma (OSCC) among FA patients is 800-folds higher than in the general population. Early detection of OSCC, preferably at it precursor stage, is critical in FA patients to improve their survival. In an ongoing clinical trial, we are evaluating the effectiveness of the programmable bio-nanochip (p-BNC)-based oral cytology test in diagnosing oral potentially malignant disorders (OPMD) in non-FA patients. We used this test to compare cytomorphometric and molecular biomarkers in OSCC cell lines derived from FA and non-FA patients to brush biopsy samples of a FA patient with OPMD and normal mucosa of healthy volunteers. Our data showed that expression patterns of molecular biomarkers were not notably different between sporadic and FA-OSCC cell lines. The p-BNC assay revealed significant differences in cytometric parameters and biomarker MCM2 expression between cytobrush samples of the FA patient and cytobrush samples of normal oral mucosa obtained from healthy volunteers. Microscopic examination of the FA patient's OPMD confirmed the presence of dysplasia. Our pilot data suggests that the p-BNC brush biopsy test recognized dysplastic oral epithelial cells in a brush biopsy sample of a FA patient.en_US
dc.identifier.citationFloriano, Pierre, Abram, Tim, Taylor, Leander, et al.. "Programmable bio-nanochip-based cytologic testing of oral potentially malignant disorders in Fanconi anemia." <i>Oral Diseases,</i> 21, no. 5 (2015) Wiley: 593-601. https://doi.org/10.1111/odi.12321.en_US
dc.identifier.doihttps://doi.org/10.1111/odi.12321en_US
dc.identifier.urihttps://hdl.handle.net/1911/94843en_US
dc.language.isoengen_US
dc.publisherWileyen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley.en_US
dc.subject.keywordFanconi anemiaen_US
dc.subject.keywordbio-nanochipen_US
dc.subject.keywordbrush biopsyen_US
dc.subject.keywordoral epithelial dysplasiaen_US
dc.subject.keywordoral potentially malignant disordersen_US
dc.subject.keywordoral squamous cell carcinomaen_US
dc.titleProgrammable bio-nanochip-based cytologic testing of oral potentially malignant disorders in Fanconi anemiaen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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