Proteomic Profiling Identifies Distinct Protein Patterns in Acute Myelogenous Leukemia CD34+CD38- Stem-Like Cells

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dc.citation.firstpagee78453
dc.citation.issueNumber10
dc.citation.journalTitlePLoS One
dc.citation.volumeNumber8
dc.contributor.authorKornblau, Steven M.
dc.contributor.authorQutub, Amina
dc.contributor.authorYao, Hui
dc.contributor.authorYork, Heather
dc.contributor.authorQiu, Yi Hua
dc.contributor.authorGraber, David
dc.contributor.authorRavandi, Farhad
dc.contributor.authorCortes, Jorge
dc.contributor.authorAndreeff, Michael
dc.contributor.authorZhang, Nianxiang
dc.contributor.authorCoombes, Kevin R.
dc.date.accessioned2013-12-20T21:02:03Z
dc.date.available2013-12-20T21:02:03Z
dc.date.issued2013
dc.description.abstractAcute myeloid leukemia (AML) is believed to arise from leukemic stem-like cells (LSC) making understanding the biological differences between LSC and normal stem cells (HSC) or common myeloid progenitors (CMP) crucial to understanding AML biology. To determine if protein expression patterns were different in LSC compared to other AML and CD34+ populations, we measured the expression of 121 proteins by Reverse Phase Protein Arrays (RPPA) in 5 purified fractions from AML marrow and blood samples: Bulk (CD3/CD19 depleted), CD34-, CD34+(CMP), CD34+CD38+ and CD34+CD38-(LSC). LSC protein expression differed markedly from Bulk (n=31 cases, 93/121 proteins) and CD34+ cells (n= 30 cases, 88/121 proteins) with 54 proteins being significantly different (31 higher, 23 lower) in LSC than in either Bulk or CD34+ cells. Sixty-seven proteins differed significantly between CD34+ and Bulk blasts (n=69 cases). Protein expression patterns in LSC and CD34+ differed markedly from normal CD34+ cells. LSC were distinct from CD34+ and Bulk cells by principal component and by protein signaling network analysis which confirmed individual protein analysis. Potential targetable submodules in LSC included the proteins PU.1(SP1), P27, Mcl1, HIF1?, cMET, P53, Yap, and phospho-Stats 1, 5 and 6. Protein expression and activation in LSC differs markedly from other blast populations suggesting that studies of AML biology should be performed in LSC.
dc.identifier.citationKornblau, Steven M., Qutub, Amina, Yao, Hui, et al.. "Proteomic Profiling Identifies Distinct Protein Patterns in Acute Myelogenous Leukemia CD34+CD38- Stem-Like Cells." <i>PLoS One,</i> 8, no. 10 (2013) Public Library of Science: e78453. http://dx.doi.org/10.1371/journal.pone.0078453.
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0078453
dc.identifier.urihttps://hdl.handle.net/1911/75308
dc.language.isoeng
dc.publisherPublic Library of Science
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleProteomic Profiling Identifies Distinct Protein Patterns in Acute Myelogenous Leukemia CD34+CD38- Stem-Like Cells
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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