Proteomic Profiling Identifies Distinct Protein Patterns in Acute Myelogenous Leukemia CD34+CD38- Stem-Like Cells

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dc.citation.firstpagee78453en_US
dc.citation.issueNumber10en_US
dc.citation.journalTitlePLoS Oneen_US
dc.citation.volumeNumber8en_US
dc.contributor.authorKornblau, Steven M.en_US
dc.contributor.authorQutub, Aminaen_US
dc.contributor.authorYao, Huien_US
dc.contributor.authorYork, Heatheren_US
dc.contributor.authorQiu, Yi Huaen_US
dc.contributor.authorGraber, Daviden_US
dc.contributor.authorRavandi, Farhaden_US
dc.contributor.authorCortes, Jorgeen_US
dc.contributor.authorAndreeff, Michaelen_US
dc.contributor.authorZhang, Nianxiangen_US
dc.contributor.authorCoombes, Kevin R.en_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2013-12-20T21:02:03Zen_US
dc.date.available2013-12-20T21:02:03Zen_US
dc.date.issued2013en_US
dc.description.abstractAcute myeloid leukemia (AML) is believed to arise from leukemic stem-like cells (LSC) making understanding the biological differences between LSC and normal stem cells (HSC) or common myeloid progenitors (CMP) crucial to understanding AML biology. To determine if protein expression patterns were different in LSC compared to other AML and CD34+ populations, we measured the expression of 121 proteins by Reverse Phase Protein Arrays (RPPA) in 5 purified fractions from AML marrow and blood samples: Bulk (CD3/CD19 depleted), CD34-, CD34+(CMP), CD34+CD38+ and CD34+CD38-(LSC). LSC protein expression differed markedly from Bulk (n=31 cases, 93/121 proteins) and CD34+ cells (n= 30 cases, 88/121 proteins) with 54 proteins being significantly different (31 higher, 23 lower) in LSC than in either Bulk or CD34+ cells. Sixty-seven proteins differed significantly between CD34+ and Bulk blasts (n=69 cases). Protein expression patterns in LSC and CD34+ differed markedly from normal CD34+ cells. LSC were distinct from CD34+ and Bulk cells by principal component and by protein signaling network analysis which confirmed individual protein analysis. Potential targetable submodules in LSC included the proteins PU.1(SP1), P27, Mcl1, HIF1?, cMET, P53, Yap, and phospho-Stats 1, 5 and 6. Protein expression and activation in LSC differs markedly from other blast populations suggesting that studies of AML biology should be performed in LSC.en_US
dc.identifier.citationKornblau, Steven M., Qutub, Amina, Yao, Hui, et al.. "Proteomic Profiling Identifies Distinct Protein Patterns in Acute Myelogenous Leukemia CD34+CD38- Stem-Like Cells." <i>PLoS One,</i> 8, no. 10 (2013) Public Library of Science: e78453. http://dx.doi.org/10.1371/journal.pone.0078453.en_US
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0078453en_US
dc.identifier.urihttps://hdl.handle.net/1911/75308en_US
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleProteomic Profiling Identifies Distinct Protein Patterns in Acute Myelogenous Leukemia CD34+CD38- Stem-Like Cellsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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