Improved high quality sand fly assemblies enabled by ultra low input long read sequencing

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dc.citation.articleNumber918en_US
dc.citation.journalTitleScientific Dataen_US
dc.citation.volumeNumber11en_US
dc.contributor.authorHuang, Michelleen_US
dc.contributor.authorKingan, Sarahen_US
dc.contributor.authorShoue, Douglasen_US
dc.contributor.authorNguyen, Oanhen_US
dc.contributor.authorFroenicke, Lutzen_US
dc.contributor.authorGalvin, Brendanen_US
dc.contributor.authorLambert, Christineen_US
dc.contributor.authorKhan, Ruqayyaen_US
dc.contributor.authorMaheshwari, Chiragen_US
dc.contributor.authorWeisz, Daviden_US
dc.contributor.authorMaslen, Garethen_US
dc.contributor.authorDavison, Helenen_US
dc.contributor.authorAiden, Erez Liebermanen_US
dc.contributor.authorKorlach, Jonasen_US
dc.contributor.authorDudchenko, Olgaen_US
dc.contributor.authorMcDowell, Mary Annen_US
dc.contributor.authorRichards, Stephenen_US
dc.contributor.orgCenter for Theoretical Biological Physicsen_US
dc.date.accessioned2024-09-10T19:29:03Zen_US
dc.date.available2024-09-10T19:29:03Zen_US
dc.date.issued2024en_US
dc.description.abstractPhlebotomine sand flies are the vectors of leishmaniasis, a neglected tropical disease. High-quality reference genomes are an important tool for understanding the biology and eco-evolutionary dynamics underpinning disease epidemiology. Previous leishmaniasis vector reference sequences were limited by sequencing technologies available at the time and inadequate for high-resolution genomic inquiry. Here, we present updated reference assemblies of two sand flies, Phlebotomus papatasi and Lutzomyia longipalpis. These chromosome-level assemblies were generated using an ultra-low input library protocol, PacBio HiFi long reads, and Hi-C technology. The new P. papatasi reference has a final assembly span of 351.6 Mb and contig and scaffold N50s of 926 kb and 111.8 Mb, respectively. The new Lu. longipalpis reference has a final assembly span of 147.8 Mb and contig and scaffold N50s of 1.09 Mb and 40.6 Mb, respectively. Benchmarking Universal Single-Copy Orthologue (BUSCO) assessments indicated 94.5% and 95.6% complete single copy insecta orthologs for P. papatasi and Lu. longipalpis. These improved assemblies will serve as an invaluable resource for future genomic work on phlebotomine sandflies.en_US
dc.identifier.citationHuang, M., Kingan, S., Shoue, D., Nguyen, O., Froenicke, L., Galvin, B., Lambert, C., Khan, R., Maheshwari, C., Weisz, D., Maslen, G., Davison, H., Aiden, E. L., Korlach, J., Dudchenko, O., McDowell, M. A., & Richards, S. (2024). Improved high quality sand fly assemblies enabled by ultra low input long read sequencing. Scientific Data, 11(1), 918. https://doi.org/10.1038/s41597-024-03628-yen_US
dc.identifier.digitals41597-024-03628-yen_US
dc.identifier.doihttps://doi.org/10.1038/s41597-024-03628-yen_US
dc.identifier.urihttps://hdl.handle.net/1911/117868en_US
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.rightsExcept where otherwise noted, this work is licensed under a Creative Commons Attribution (CC BY) license.  Permission to reuse, publish, or reproduce the work beyond the terms of the license or beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleImproved high quality sand fly assemblies enabled by ultra low input long read sequencingen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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