Metabolic transistor strategy for controlling electron transfer chain activity in Escherichia coli

dc.citation.firstpage159en_US
dc.citation.journalTitleMetabolic Engineeringen_US
dc.citation.lastpage168en_US
dc.citation.volumeNumber28en_US
dc.contributor.authorWu, Huien_US
dc.contributor.authorTuli, Leepikaen_US
dc.contributor.authorBennett, George N.en_US
dc.contributor.authorSan, Ka-Yiuen_US
dc.date.accessioned2015-02-11T15:45:10Zen_US
dc.date.available2015-02-11T15:45:10Zen_US
dc.date.issued2015en_US
dc.description.abstractA novel strategy to finely control a large metabolic flux by using a “metabolic transistor” approach was established. In this approach a small change in the level or availability of an essential component for the process is controlled by adding a competitive reaction that affects a precursor or an intermediate in its biosynthetic pathway. The change of the basal level of the essential component, considered as a base current in a transistor, has a large effect on the flux through the major pathway. In this way, the fine-tuning of a large flux can be accomplished. The “metabolic transistor” strategy was applied to control electron transfer chain function by manipulation of the quinone synthesis pathway in Escherichia coli. The achievement of a theoretical yield of lactate production under aerobic conditions via this strategy upon manipulation of the biosynthetic pathway of the key participant, ubiquinone-8 (Q8), in an E. coli strain provides an in vivo, genetically tunable means to control the activity of the electron transfer chain and manipulate the production of reduced products while limiting consumption of oxygen to a defined amount.en_US
dc.identifier.citationWu, Hui, Tuli, Leepika, Bennett, George N., et al.. "Metabolic transistor strategy for controlling electron transfer chain activity in Escherichia coli." <i>Metabolic Engineering,</i> 28, (2015) Elsevier: 159-168. http://dx.doi.org/10.1016/j.ymben.2015.01.002.en_US
dc.identifier.doihttp://dx.doi.org/10.1016/j.ymben.2015.01.002en_US
dc.identifier.urihttps://hdl.handle.net/1911/79028en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.en_US
dc.subject.keywordEscherichia colien_US
dc.subject.keywordmetabolic transistoren_US
dc.subject.keywordelectron transfer chainen_US
dc.subject.keywordquinoneen_US
dc.subject.keywordlactateen_US
dc.subject.keywordoxygenen_US
dc.titleMetabolic transistor strategy for controlling electron transfer chain activity in Escherichia colien_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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