Understanding the Role of Protein Degradation in Synthetic Gene Circuits

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dc.contributor.advisorBennett, Matthew Ren_US
dc.creatorO'Brien, Erin Oen_US
dc.date.accessioned2019-05-17T15:21:45Zen_US
dc.date.available2019-05-17T15:21:45Zen_US
dc.date.created2018-05en_US
dc.date.issued2018-04-20en_US
dc.date.submittedMay 2018en_US
dc.date.updated2019-05-17T15:21:45Zen_US
dc.description.abstractSynthetic gene circuits are built with mathematical predictions and are further characterized experimentally. Most synthetic circuits utilize degradation tags to normalize and speed up the rate of degradation of circuit components. Despite their widespread use, the effect of degradation tags on circuit dynamics has not been well studied. This work aims to characterize the degradation rate of the ssrA degradation tag variants on a single substrate level and determine their role in overall network dynamics within a synthetic gene circuit at the single cell resolution. mall differences in the protein degradation rates indicating that the parameter space for the degradation tags is critical for achieving desired circuit dynamics. Mathematically and experimentally, this work demonstrates varying the rate of degradation can ultimately dictate the output oscillations for the circuit dynamics. The ultimate goal of this work is to create a better understanding of the role degradation plays in synthetic gene circuit dynamics.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationO'Brien, Erin O. "Understanding the Role of Protein Degradation in Synthetic Gene Circuits." (2018) Diss., Rice University. <a href="https://hdl.handle.net/1911/105771">https://hdl.handle.net/1911/105771</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/105771en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectSynthetic Biologyen_US
dc.subjectssrAen_US
dc.subjectClpXPen_US
dc.subjectProtein Degradationen_US
dc.subjectMathematical Modelingen_US
dc.titleUnderstanding the Role of Protein Degradation in Synthetic Gene Circuitsen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBiochemistry and Cell Biologyen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelDoctoralen_US
thesis.degree.majorSynthetic Biologyen_US
thesis.degree.nameDoctor of Philosophyen_US
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