Development, characterization, and applications of multi-material stereolithography bioprinting

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dc.citation.articleNumber3171en_US
dc.citation.journalTitleScientific Reportsen_US
dc.citation.volumeNumber11en_US
dc.contributor.authorGrigoryan, Bagraten_US
dc.contributor.authorSazer, Daniel W.en_US
dc.contributor.authorAvila, Amandaen_US
dc.contributor.authorAlbritton, Jacob L.en_US
dc.contributor.authorPadhye, Aparnaen_US
dc.contributor.authorTa, Anderson H.en_US
dc.contributor.authorGreenfield, Paul T.en_US
dc.contributor.authorGibbons, Don L.en_US
dc.contributor.authorMiller, Jordan S.en_US
dc.date.accessioned2021-02-24T19:16:06Zen_US
dc.date.available2021-02-24T19:16:06Zen_US
dc.date.issued2021en_US
dc.description.abstractAs a 3D bioprinting technique, hydrogel stereolithography has historically been limited in its ability to capture the spatial heterogeneity that permeates mammalian tissues and dictates structure–function relationships. This limitation stems directly from the difficulty of preventing unwanted material mixing when switching between different liquid bioinks. Accordingly, we present the development, characterization, and application of a multi-material stereolithography bioprinter that provides controlled material selection, yields precise regional feature alignment, and minimizes bioink mixing. Fluorescent tracers were first used to highlight the broad design freedoms afforded by this fabrication strategy, complemented by morphometric image analysis to validate architectural fidelity. To evaluate the bioactivity of printed gels, 344SQ lung adenocarcinoma cells were printed in a 3D core/shell architecture. These cells exhibited native phenotypic behavior as evidenced by apparent proliferation and formation of spherical multicellular aggregates. Cells were also printed as pre-formed multicellular aggregates, which appropriately developed invasive protrusions in response to hTGF-β1. Finally, we constructed a simplified model of intratumoral heterogeneity with two separate sub-populations of 344SQ cells, which together grew over 14 days to form a dense regional interface. Together, these studies highlight the potential of multi-material stereolithography to probe heterotypic interactions between distinct cell types in tissue-specific microenvironments.en_US
dc.identifier.citationGrigoryan, Bagrat, Sazer, Daniel W., Avila, Amanda, et al.. "Development, characterization, and applications of multi-material stereolithography bioprinting." <i>Scientific Reports,</i> 11, (2021) Springer Nature: https://doi.org/10.1038/s41598-021-82102-w.en_US
dc.identifier.digitals41598-021-82102-wen_US
dc.identifier.doihttps://doi.org/10.1038/s41598-021-82102-wen_US
dc.identifier.urihttps://hdl.handle.net/1911/110104en_US
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleDevelopment, characterization, and applications of multi-material stereolithography bioprintingen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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