Tissue Engineered, Hydrogel-Based Endothelial Progenitor Cell Therapy Robustly Revascularizes Ischemic Myocardium and Preserves Ventricular Function

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dc.citation.firstpage1090
dc.citation.issueNumber3
dc.citation.journalTitleThe Journal of Thoracic and Cardiovascular Surgery
dc.citation.lastpage1098
dc.citation.volumeNumber148
dc.contributor.authorAtluri, Pavan
dc.contributor.authorMiller, Jordan S.
dc.contributor.authorEmery, Robert J.
dc.contributor.authorHung, George
dc.contributor.authorTrubelja, Alen
dc.contributor.authorCohen, Jeffrey E.
dc.contributor.authorLloyd, Kelsey
dc.contributor.authorHan, Jason
dc.contributor.authorGaffey, Ann C.
dc.contributor.authorMacArthur, John W.
dc.contributor.authorChen, Christopher S.
dc.contributor.authorWoo, Y. Joseph
dc.date.accessioned2015-10-28T19:04:03Z
dc.date.available2015-10-28T19:04:03Z
dc.date.issued2014
dc.description.abstractObjectives: Cell-based angiogenic therapy for ischemic heart failure has had limited clinical impact, likely related to low cell retention (<1%) and dispersion. We developed a novel, tissue-engineered, hydrogel-based cell-delivery strategy to overcome these limitations and provide prolonged regional retention of myocardial endothelial progenitor cells at high cell dosage. Methods: Endothelial progenitor cells were isolated from Wistar rats and encapsulated in fibrin gels. In vitro viability was quantified using a fluorescent live-dead stain of transgenic enhanced green fluorescent protein+ endothelial progenitor cells. Endothelial progenitor cell-laden constructs were implanted onto ischemic rat myocardium in a model of acute myocardial infarction (left anterior descending ligation) for 4 weeks. Intramyocardial cell injection (2 × 106 endothelial progenitor cells), empty fibrin, and isolated left anterior descending ligation groups served as controls. Hemodynamics were quantified using echocardiography, Doppler flow analysis, and intraventricular pressure-volume analysis. Vasculogenesis and ventricular geometry were quantified. Endothelial progenitor cell migration was analyzed by using endothelial progenitor cells from transgenic enhanced green fluorescent protein+ rodents. Results: Endothelial progenitor cells demonstrated an overall 88.7% viability for all matrix and cell conditions investigated after 48 hours. Histologic assessment of 1-week implants demonstrated significant migration of transgenic enhanced green fluorescent protein+ endothelial progenitor cells from the fibrin matrix to the infarcted myocardium compared with intramyocardial cell injection (28 ± 12.3 cells/high power field vs 2.4 ± 2.1 cells/high power field, P = .0001). We also observed a marked increase in vasculogenesis at the implant site. Significant improvements in ventricular hemodynamics and geometry were present after endothelial progenitor cell-hydrogel therapy compared with control. Conclusions: We present a tissue-engineered, hydrogel-based endothelial progenitor cell-mediated therapy to enhance cell delivery, cell retention, vasculogenesis, and preservation of myocardial structure and function.
dc.identifier.citationAtluri, Pavan, Miller, Jordan S., Emery, Robert J., et al.. "Tissue Engineered, Hydrogel-Based Endothelial Progenitor Cell Therapy Robustly Revascularizes Ischemic Myocardium and Preserves Ventricular Function." <i>The Journal of Thoracic and Cardiovascular Surgery,</i> 148, no. 3 (2014) Elsevier: 1090-1098. http://dx.doi.org/10.1016/j.jtcvs.2014.06.038.
dc.identifier.doihttp://dx.doi.org/10.1016/j.jtcvs.2014.06.038
dc.identifier.urihttps://hdl.handle.net/1911/81938
dc.language.isoeng
dc.publisherElsevier
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
dc.subject.keywordbioengineering
dc.subject.keywordhydrogel
dc.subject.keywordEndothelial Progenitor Cell
dc.subject.keywordvasculogenesis
dc.subject.keywordischemic cardiomyopathy
dc.titleTissue Engineered, Hydrogel-Based Endothelial Progenitor Cell Therapy Robustly Revascularizes Ischemic Myocardium and Preserves Ventricular Function
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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