Evolution of the activation domain in a Hox transcription factor

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dc.citation.firstpage745en_US
dc.citation.journalTitleThe International Journal of Developmental Biologyen_US
dc.citation.lastpage753en_US
dc.citation.volumeNumber62en_US
dc.contributor.authorLiu, Yingen_US
dc.contributor.authorHuang, Annieen_US
dc.contributor.authorBooth, Rebecca M.en_US
dc.contributor.authorMendes, Gabriela Geraldoen_US
dc.contributor.authorMerchant, Zabeenaen_US
dc.contributor.authorMatthews, Kathleen S.en_US
dc.contributor.authorBondos, Sarah E.en_US
dc.date.accessioned2019-01-18T17:51:30Zen_US
dc.date.available2019-01-18T17:51:30Zen_US
dc.date.issued2018en_US
dc.description.abstractLinking changes in amino acid sequences to the evolution of transcription regulatory domains is often complicated by the low sequence complexity and high mutation rates of intrinsically disordered protein regions. For the Hox transcription factor Ultrabithorax (Ubx), conserved motifs distributed throughout the protein sequence enable direct comparison of specific protein regions, despite variations in the length and composition of the intervening sequences. In cell culture, the strength of transcription activation by Drosophila melanogaster Ubx correlates with the presence of a predicted helix within its activation domain. Curiously, this helix is not preserved in species more divergent than flies, suggesting the nature of transcription activation may have evolved. To determine whether this helix contributes to Drosophila Ubx function in vivo, wild-type and mutant proteins were ectopically expressed in the developing wing and the phenotypes evaluated. Helix mutations alter Drosophila Ubx activity in the developing wing, demonstrating its functional importance in vivo. The locations of activation domains in Ubx orthologues were identified by testing the ability of truncation mutants to activate transcription in yeast one-hybrid assays. In Ubx orthologues representing 540 million years of evolution, the ability to activate transcription varies substantially. The sequence and the location of the activation domains also differ. Consequently, analogous regions of Ubx orthologues change function over time, and may activate transcription in one species, but have no activity, or even inhibit transcription activation in another species. Unlike homeodomain-DNA binding, the nature of transcription activation by Ubx has substantially evolved.en_US
dc.identifier.citationLiu, Ying, Huang, Annie, Booth, Rebecca M., et al.. "Evolution of the activation domain in a Hox transcription factor." <i>The International Journal of Developmental Biology,</i> 62, (2018) UPV/EHU Press: 745-753. https://doi.org/10.1387/ijdb.180151sb.en_US
dc.identifier.doihttps://doi.org/10.1387/ijdb.180151sben_US
dc.identifier.urihttps://hdl.handle.net/1911/105106en_US
dc.language.isoengen_US
dc.publisherUPV/EHU Pressen_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.subject.keywordintrinsic disorderen_US
dc.subject.keywordnatively unfoldeden_US
dc.subject.keywordUltrabithoraxen_US
dc.subject.keywordUbxen_US
dc.subject.keywordevo-devoen_US
dc.titleEvolution of the activation domain in a Hox transcription factoren_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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