Injectable OPF/graphene oxide hydrogels provide mechanical support and enhance cell electrical signaling after implantation into myocardial infarct

Simple item page
dc.citation.firstpage3317en_US
dc.citation.issueNumber12en_US
dc.citation.journalTitleTheranosticsen_US
dc.citation.lastpage3330en_US
dc.citation.volumeNumber8en_US
dc.contributor.authorZhou, Jinen_US
dc.contributor.authorYang, Xiaoningen_US
dc.contributor.authorLiu, Weien_US
dc.contributor.authorWang, Chunlanen_US
dc.contributor.authorShen, Yuanen_US
dc.contributor.authorZhang, Fengzhien_US
dc.contributor.authorZhu, Huiminen_US
dc.contributor.authorSun, Hongjien_US
dc.contributor.authorChen, Jiayunen_US
dc.contributor.authorLam, Johnnyen_US
dc.contributor.authorMikos, Antonios G.en_US
dc.contributor.authorWang, Changyongen_US
dc.date.accessioned2018-09-26T14:52:44Zen_US
dc.date.available2018-09-26T14:52:44Zen_US
dc.date.issued2018en_US
dc.description.abstractAfter myocardial infarction (MI), the scar tissue contributes to ventricular dysfunction by electrically uncoupling viable cardiomyocytes in the infarct region. Injection of a conductive hydrogel could not only provide mechanical support to the infarcted region, but also synchronize contraction and restore ventricular function by electrically connecting isolated cardiomyocytes to intact tissue. Methods: We created a conductive hydrogel by introducing graphene oxide (GO) nanoparticles into oligo(poly(ethylene glycol) fumarate) (OPF) hydrogels. The hydrogels were characterized by AFM and electrochemistry workstation. A rat model of myocardial infarction was used to investigate the ability of OPF/GO to improve cardiac electrical propagation in the injured heart in vivo. Echocardiography (ECHO) was used to evaluate heart function 4 weeks after MI. Ca2+ imaging was used to visualize beating cardiomyocytes (CMs). Immunofluorescence staining was used to visualize the expression of cardiac-specific markers. Results: OPF/GO hydrogels had semiconductive properties that were lacking in pure OPF. In addition, the incorporation of GO into OPF hydrogels could improve cell attachment in vitro. Injection of OPF/GO 4 weeks after myocardial infarction in rats enhanced the Ca2+ signal conduction of cardiomyocytes in the infarcted region in comparison with PBS or OPF alone. Moreover, the injection of OPF/GO hydrogel into the infarct region enhanced the generation of cytoskeletal structure and intercalated disc assembly. Echocardiography analysis showed improvement in load-dependent ejection fraction/fractional shortening of heart function 4 weeks after injection. Conclusions: We prepared a conductive hydrogel (OPF/GO) that provide mechanical support and biological conduction in vitro and in vivo. We found that injected OPF/GO hydrogels can provide mechanical support and electric connection between healthy myocardium and the cardiomyocytes in the scar via activating the canonical Wnt signal pathway, thus upregulating the generation of Cx43 and gap junction associated proteins. Injection of OPF/GO hydrogel maintained better heart function after myocardial infarction than the injection of a nonconductive polymer.en_US
dc.identifier.citationZhou, Jin, Yang, Xiaoning, Liu, Wei, et al.. "Injectable OPF/graphene oxide hydrogels provide mechanical support and enhance cell electrical signaling after implantation into myocardial infarct." <i>Theranostics,</i> 8, no. 12 (2018) Ivyspring: 3317-3330. https://doi.org/10.7150/thno.25504.en_US
dc.identifier.digitalv08p3317en_US
dc.identifier.doihttps://doi.org/10.7150/thno.25504en_US
dc.identifier.urihttps://hdl.handle.net/1911/102719en_US
dc.language.isoengen_US
dc.publisherIvyspringen_US
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/).en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subject.keywordconductionen_US
dc.subject.keywordinjectable biomaterialsen_US
dc.subject.keywordmyocardial infarctionen_US
dc.subject.keywordremodelingen_US
dc.titleInjectable OPF/graphene oxide hydrogels provide mechanical support and enhance cell electrical signaling after implantation into myocardial infarcten_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
v08p3317.pdf
Size:
3.33 MB
Format:
Adobe Portable Document Format
Download
Collections
Faculty Publications
Bioengineering Publications