Antioxidant Carbon Nanoparticles Inhibit Fibroblast-Like Synoviocyte Invasiveness and Reduce Disease Severity in a Rat Model of Rheumatoid Arthritis

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dc.citation.articleNumber1005en_US
dc.citation.issueNumber10en_US
dc.citation.journalTitleAntioxidantsen_US
dc.citation.volumeNumber9en_US
dc.contributor.authorTanner, Mark R.en_US
dc.contributor.authorHuq, Redwanen_US
dc.contributor.authorSikkema, William K.A.en_US
dc.contributor.authorNilewski, Lizanne G.en_US
dc.contributor.authorYosef, Nejlaen_US
dc.contributor.authorSchmitt, Codyen_US
dc.contributor.authorFlores-Suarez, Carlos P.en_US
dc.contributor.authorRaugh, Arielleen_US
dc.contributor.authorLaragione, Teresinaen_US
dc.contributor.authorGulko, Pércio S.en_US
dc.contributor.authorTour, James M.en_US
dc.contributor.authorBeeton, Christineen_US
dc.contributor.orgThe NanoCarbon Centeren_US
dc.date.accessioned2020-11-06T02:02:48Zen_US
dc.date.available2020-11-06T02:02:48Zen_US
dc.date.issued2020en_US
dc.description.abstractReactive oxygen species have been involved in the pathogenesis of rheumatoid arthritis (RA). Our goal was to determine the effects of selectively scavenging superoxide (O2•−) and hydroxyl radicals with antioxidant nanoparticles, called poly(ethylene glycol)-functionalized hydrophilic carbon clusters (PEG-HCCs), on the pathogenic functions of fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA) and on the progression of an animal model of RA. We used human FLS from patients with RA to determine PEG-HCC internalization and effects on FLS cytotoxicity, invasiveness, proliferation, and production of proteases. We used the pristane-induced arthritis (PIA) rat model of RA to assess the benefits of PEG-HCCs on reducing disease severity. PEG-HCCs were internalized by RA-FLS, reduced their intracellular O2•−, and reduced multiple measures of their pathogenicity in vitro, including proliferation and invasion. In PIA, PEG-HCCs caused a 65% reduction in disease severity, as measured by a standardized scoring system of paw inflammation and caused a significant reduction in bone and tissue damage, and circulating rheumatoid factor. PEG-HCCs did not induce lymphopenia during PIA. Our study demonstrated a role for O2•− and hydroxyl radicals in the pathogenesis of a rat model of RA and showed efficacy of PEG-HCCs in treating a rat model of RA.en_US
dc.identifier.citationTanner, Mark R., Huq, Redwan, Sikkema, William K.A., et al.. "Antioxidant Carbon Nanoparticles Inhibit Fibroblast-Like Synoviocyte Invasiveness and Reduce Disease Severity in a Rat Model of Rheumatoid Arthritis." <i>Antioxidants,</i> 9, no. 10 (2020) MDPI: https://doi.org/10.3390/antiox9101005.en_US
dc.identifier.doihttps://doi.org/10.3390/antiox9101005en_US
dc.identifier.urihttps://hdl.handle.net/1911/109516en_US
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly citeden_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subject.keywordsynovial fibroblasten_US
dc.subject.keywordoxidative stressen_US
dc.subject.keywordnanomaterialsen_US
dc.titleAntioxidant Carbon Nanoparticles Inhibit Fibroblast-Like Synoviocyte Invasiveness and Reduce Disease Severity in a Rat Model of Rheumatoid Arthritisen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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