Electronic control of redox reactions inside Escherichia coli using a genetic module

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dc.citation.articleNumbere0258380en_US
dc.citation.issueNumber11en_US
dc.citation.journalTitlePLOS ONEen_US
dc.citation.volumeNumber16en_US
dc.contributor.authorBaruch, Mosheen_US
dc.contributor.authorTejedor-Sanz, Saraen_US
dc.contributor.authorSu, Linen_US
dc.contributor.authorAjo-Franklin, Caroline M.en_US
dc.contributor.orgInstitute for Biosciences and Bioengineeringen_US
dc.date.accessioned2021-12-02T15:09:24Zen_US
dc.date.available2021-12-02T15:09:24Zen_US
dc.date.issued2021en_US
dc.description.abstractMicroorganisms regulate the redox state of different biomolecules to precisely control biological processes. These processes can be modulated by electrochemically coupling intracellular biomolecules to an external electrode, but current approaches afford only limited control and specificity. Here we describe specific electrochemical control of the reduction of intracellular biomolecules in Escherichia coli through introduction of a heterologous electron transfer pathway. E. coli expressing cymAmtrCAB from Shewanella oneidensis MR-1 consumed electrons directly from a cathode when fumarate or nitrate, both intracellular electron acceptors, were present. The fumarate-triggered current consumption occurred only when fumarate reductase was present, indicating all the electrons passed through this enzyme. Moreover, CymAMtrCAB-expressing E. coli used current to stoichiometrically reduce nitrate. Thus, our work introduces a modular genetic tool to reduce a specific intracellular redox molecule with an electrode, opening the possibility of electronically controlling biological processes such as biosynthesis and growth in any microorganism.en_US
dc.identifier.citationBaruch, Moshe, Tejedor-Sanz, Sara, Su, Lin, et al.. "Electronic control of redox reactions inside Escherichia coli using a genetic module." <i>PLOS ONE,</i> 16, no. 11 (2021) Public Library of Science: https://doi.org/10.1371/journal.pone.0258380.en_US
dc.identifier.digitaljournal-pone-0258380en_US
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0258380en_US
dc.identifier.urihttps://hdl.handle.net/1911/111726en_US
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleElectronic control of redox reactions inside Escherichia coli using a genetic moduleen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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