Loop dynamics of thymidine diphosphate-rhamnose 3′-O-methyltransferase (CalS11), an enzyme in calicheamicin biosynthesis

Simple item page
dc.citation.articleNumber12004en_US
dc.citation.issueNumber1en_US
dc.citation.journalTitleStructural Dynamicsen_US
dc.citation.volumeNumber3en_US
dc.contributor.authorHan, Luen_US
dc.contributor.authorSingh, Shanterien_US
dc.contributor.authorThorson, Jon S.en_US
dc.contributor.authorPhillips, George N.Jr.en_US
dc.date.accessioned2017-05-22T21:48:43Zen_US
dc.date.available2017-05-22T21:48:43Zen_US
dc.date.issued2016en_US
dc.description.abstractStructure analysis and ensemble refinement of the apo-structure of thymidine diphosphate (TDP)-rhamnose 3′-O-methyltransferase reveal a gate for substrate entry and product release. TDP-rhamnose 3′-O-methyltransferase (CalS11) catalyses a 3′-O-methylation of TDP-rhamnose, an intermediate in the biosynthesis of enediyne antitumor antibiotic calicheamicin. CalS11 operates at the sugar nucleotide stage prior to glycosylation step. Here, we present thecrystal structure of the apo form of CalS11 at 1.89 Å resolution. We propose that the L2 loop functions as a gate facilitating and/or providing specificity for substrate entry or promoting product release. Ensemble refinement analysis slightly improves the crystallographic refinement statistics and furthermore provides a compelling way to visualize the dynamic model of loop L2, supporting the understanding of its proposed role in catalysis.en_US
dc.identifier.citationHan, Lu, Singh, Shanteri, Thorson, Jon S., et al.. "Loop dynamics of thymidine diphosphate-rhamnose 3′-O-methyltransferase (CalS11), an enzyme in calicheamicin biosynthesis." <i>Structural Dynamics,</i> 3, no. 1 (2016) AIP Publishing: http://dx.doi.org/10.1063/1.4941368.en_US
dc.identifier.doihttp://dx.doi.org/10.1063/1.4941368en_US
dc.identifier.urihttps://hdl.handle.net/1911/94348en_US
dc.language.isoengen_US
dc.publisherAIP Publishingen_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.titleLoop dynamics of thymidine diphosphate-rhamnose 3′-O-methyltransferase (CalS11), an enzyme in calicheamicin biosynthesisen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Loop-dynamics.pdf
Size:
1.59 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Faculty Publications
BioSciences Publications
Chemistry Publications