Diversity Metrics Are Robust to Differences in Sampling Location and Depth for Environmental DNA of Plants in Small Temperate Lakes

Simple item page
dc.citation.articleNumber617924en_US
dc.citation.journalTitleFrontiers in Environmental Scienceen_US
dc.citation.volumeNumber9en_US
dc.contributor.authorDrummond, Jennifer A.en_US
dc.contributor.authorLarson, Eric R.en_US
dc.contributor.authorLi, Yiyuanen_US
dc.contributor.authorLodge, David M.en_US
dc.contributor.authorGantz, Crysta A.en_US
dc.contributor.authorPfrender, Michael E.en_US
dc.contributor.authorRenshaw, Mark A.en_US
dc.contributor.authorCorrea, Adrienne M.S.en_US
dc.contributor.authorEgan, Scott P.en_US
dc.date.accessioned2021-06-17T19:01:30Zen_US
dc.date.available2021-06-17T19:01:30Zen_US
dc.date.issued2021en_US
dc.description.abstractEnvironmental DNA (eDNA) analysis methods permit broad yet detailed biodiversity sampling to be performed with minimal field effort. However, considerable uncertainty remains regarding the spatial resolution necessary for effective sampling, especially in aquatic environments. Also, contemporary plant communities are under-investigated with eDNA methods relative to animals and microbes. We analyzed eDNA samples from six small temperate lakes to elucidate spatial patterns in the distributions of algae and aquatic and terrestrial plants, using metabarcoding of the Internal Transcribed Spacer-1 (ITS1) genomic region. Sampling locations were varied across horizontal and vertical space: sites in each lake included a mixture of nearshore and offshore positions, each of which was stratified into surface (shallow) and benthic (deep) samples. We detected the expected community variation (beta diversity) from lake to lake, but only small effects of offshore distance and sampling depth. Taxon richness (alpha diversity) was slightly elevated in nearshore samples, but displayed no other significant spatial effects. These diversity metrics imply that plant eDNA is more evenly distributed than its generating organisms in these small lake environments. Read abundances were heavily weighted toward aquatic macrophytes, though taxon richness was greatest in the algae and other nonvascular plants. We also identified representatives of many phylogenetically and ecologically varied plant taxa, including terrestrial species from surrounding areas. We conclude that freshwater plant eDNA surveys successfully capture differences among lake communities, and that easily-accessible, shore-based sampling may be a reliable technique for informing research and management in similar ecosystems.en_US
dc.identifier.citationDrummond, Jennifer A., Larson, Eric R., Li, Yiyuan, et al.. "Diversity Metrics Are Robust to Differences in Sampling Location and Depth for Environmental DNA of Plants in Small Temperate Lakes." <i>Frontiers in Environmental Science,</i> 9, (2021) Frontiers Media S.A.: https://doi.org/10.3389/fenvs.2021.617924.en_US
dc.identifier.digitalfenvs-09-617924en_US
dc.identifier.doihttps://doi.org/10.3389/fenvs.2021.617924en_US
dc.identifier.urihttps://hdl.handle.net/1911/110742en_US
dc.language.isoengen_US
dc.publisherFrontiers Media S.A.en_US
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleDiversity Metrics Are Robust to Differences in Sampling Location and Depth for Environmental DNA of Plants in Small Temperate Lakesen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
fenvs-09-617924.pdf
Size:
1.9 MB
Format:
Adobe Portable Document Format
Download
Collections
Faculty Publications
BioSciences Publications